Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium

2016 ◽  
pp. 445-456 ◽  
Author(s):  
Patrizia Danieli ◽  
Giuseppe Malpasso ◽  
Maria Chiara Ciuffreda ◽  
Massimiliano Gnecchi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Human Mesenchymal Stem Cells

Human mesenchymal stem cells-conditioned medium improves diabetic wound healing mainly through modulating fibroblast behaviors

2019 ◽  
Vol 312 (5) ◽  
pp. 325-336 ◽  
Author(s):  
Mona Saheli ◽  
Mohammad Bayat ◽  
Rasoul Ganji ◽  
Farzane Hendudari ◽  
Raziyeh Kheirjou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Human Mesenchymal Stem Cells ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

Analysis of the Effects of Low-Dose Radiation on Human Mesenchymal Stem Cells

2021 ◽  
Vol 65 (6) ◽  
pp. 5-10
Author(s):  
D. Usupzhanova ◽  
T. Astrelina ◽  
I. Kobzeva ◽  
V. Nikitina ◽  
Yu. Suchkova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Adaptive Response ◽  
Proliferative Activity ◽  
Human Mesenchymal Stem Cells ◽  
Conditioned Media ◽  
X Ray ◽  
Cultivation In Vitro

Purpose: The aim of the study was to study the effect of low X-ray doses on human mesenchymal stem cells (MSCs) in long-term cultivation in vitro. Material and methods: MSCs of the mucosal gum tissue of human were used. Cells were irradiated using an RUST-M1 X-ray unit (Russia) at doses of 50, 80, 100, 250 and 1000 mGy (dose rate40 mGy/min) and then cultivated according to standard methods. Immunological characteristics and viability of MSCs were evaluated on a FACSCanto II flow cytometer (Becton Dickinson CA, USA) for early and late passengers. Proliferative activity (PA) was evaluated using an xCelligence real-time cell analyzer (ACEA Biosciencs, Inc.). Results: It was shown that the proliferative activity (PA) of MSCs of the mucosal gum tissue which were irradiated at dose 50 mGy is comparable with the control group in long-term cultivation while doses of 100 and 250 mGy showed a decrease of PA. Also non-irradiated MSCs showed a significant decrease of the PA during cultivation in a conditioned medium from cells that received dose of 1000 mGy and an increase of PA during cultivation in a conditioned medium of cells that received doses of 50, 100 and 250 mGy. The cells were previously irradiated at dose 250 mGy showed adaptive response during cultivation in conditioned medium from cells that received dose of 1000 mGy. Сonclusion: The assessment of the effects of low radiation doses was focused on the bystander effect in the presented study. It was noted after adding conditioned media from irradiated cells to previously irradiated and non-irradiated MSCs. The bystander effects for low and high doses are different and their biological meaning requires further study. The phenomenon of adaptive response was shown after addition conditioned media from cells irradiated at dose 1000 mGy to pre-irradiated MSCs received a dose of 250 mGy. The obtained result leads to the conclusion that the effects of low doses can be positive. Thus, the results of study mainly correspond to the threshold nonlinear concept, according to which the effect is not proportional to the received radiation dose.

scholarly journals Osteogenic differentiation of human mesenchymal stem cells synergistically enhanced by biomimetic peptide amphiphiles combined with conditioned medium

2011 ◽  
Vol 7 (2) ◽  
pp. 675-682 ◽  
Author(s):  
Joel M. Anderson ◽  
Jeremy B. Vines ◽  
Jessica L. Patterson ◽  
Haiyan Chen ◽  
Amjad Javed ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Conditioned Medium ◽  
Human Mesenchymal Stem Cells ◽  
Peptide Amphiphiles

Human mesenchymal stem cells differentiate into keratocyte-like cells in keratocyte-conditioned medium

2012 ◽  
Vol 101 ◽  
pp. 16-26 ◽  
Author(s):  
Soo Hyun Park ◽  
Kyoung Woo Kim ◽  
Yeoun Sook Chun ◽  
Jae Chan Kim
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Human Mesenchymal Stem Cells

Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay

2017 ◽  
Vol 55 (3) ◽  
pp. 2285-2300 ◽  
Author(s):  
Eliana Baez-Jurado ◽  
Gina Guio Vega ◽  
Gjumrakch Aliev ◽  
Vadim V. Tarasov ◽  
Paula Esquinas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Human Mesenchymal Stem Cells ◽  
Scratch Assay ◽  
Human Astrocyte

scholarly journals 484 CATABOLIC FACTORS AND OSTEOARTHRITIS-CONDITIONED MEDIUM INHIBIT CHONDROGENESIS OF HUMAN MESENCHYMAL STEM CELLS

2008 ◽  
Vol 16 ◽  
pp. S209
Author(s):  
G. Heldens ◽  
E. Vitters ◽  
W. Schreurs ◽  
E. Piek ◽  
W. van den Berg ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Human Mesenchymal Stem Cells
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