A Semiempirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis

2016 ◽  
pp. 21-35 ◽  
Author(s):  
Philippe Schmitt-Kopplin ◽  
Agnes Fekete
Keyword(s):  
Small Molecules ◽  
Comprehensive Evaluation ◽  
Free Zone ◽  
Semiempirical Approach ◽  
Zone Electrophoresis

Free Zone Electrophoresis of Clotting Factors

1979 ◽  
Author(s):  
T. Cartwright ◽  
C.H. Dickerson ◽  
D.E.G. Austen
Keyword(s):  
Factor Viii ◽  
Total Yield ◽  
Solid Matrix ◽  
Clotting Factors ◽  
Free Zone ◽  
Zone Electrophoresis ◽  
Flow Apparatus ◽  
Electrophoresis System ◽  
Flow Through ◽  
Set Up

A free-zone electrophoresis system is used for rapid production of substantially purified factor VIII from either citrated plasma or from a factor VIII Concentrate of intermediate purety. Using concentrate, a total yield of 43% of clotting activity is obtained with fibrinogen reduced to approximately 30% of its starting level. Protein level is reduced to 20% of the original value. Using plasma, the albumin, factor VIII and fibrinogen were all separated from factor VIII. In these experiments, factor-VIII-related antigen migrates with the coagulant factor VIII illustrating that both have similar mobilities equivalent to that of an alpha-globulin, or else that they are connected together. The particular advantage of this separation is that it is carried out in a continuous flow apparatus in liquid buffer with no solid matrix and that once the apparatus is set up, material can flow through the machine at a rate of 5 mls/min being continuously separated. The main problem and area for further study is that of concentrating the relatively dilute fractions without undue loss.

Factors affecting free zone electrophoresis and isoelectric focusing in capillary electrophoresis

1989 ◽  
Vol 480 ◽  
pp. 311-319 ◽  
Author(s):  
Mingde Zhu ◽  
David L. Hansen ◽  
Sam Burd ◽  
Frank Gannon
Keyword(s):  
Capillary Electrophoresis ◽  
Isoelectric Focusing ◽  
Factors Affecting ◽  
Free Zone ◽  
Zone Electrophoresis

Highly Purified Foot-and-Mouth Disease Virus: Optical and Biological Measurements during Zone Electrophoresis in a Glucose Density Gradient

1966 ◽  
Vol 21 (8) ◽  
pp. 774-782 ◽  
Author(s):  
H. D. Matheka ◽  
H. L. Bachrach ◽  
R. Trautman
Keyword(s):  
Density Gradient ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Optical Methods ◽  
High Passage ◽  
Free Zone ◽  
Mouth Disease Virus ◽  
Zone Electrophoresis ◽  
Foot And Mouth

Highly purified, concentrated foot-and-mouth disease virus, type A, was homogeneous and monodisperse by carrier-free zone electrophoresis in a glucose density gradient. Virus mobilities in veronal-acetate buffer, pH 8.6 and ionic strength 0.1, were identical whether determined by infectivity or optical methods. Low passage calf-kidney virus had a significantly higher mobility, 3.28 × 10-5 cm2/volt · sec, than high passage virus, 2.84 × 10-5 cm2/volt · sec. Single passages of the high passage virus in baby hamster kidney cells did not change its mobility. Regardless of passage history, storage at 4°C in 0.05 M phosphate at pH 7.5 increased mobilities to about 3.75 × 10-5 cm2/volt · sec with retention of infectivity; some virions broke down to protein and ribonucleic acid. These electrophoretically distinct viruses possessed different absorbance-temperature profiles but identical sedimentation constants.

Carrier-free zone electrophoresis, displacement electrophoresis and isoelectric focusing in a high-performance electrophoresis apparatus

1987 ◽  
Vol 403 ◽  
pp. 47-61 ◽  
Author(s):  
Stellan Hjertén ◽  
Karin Elenbring ◽  
Ferenc Kilár ◽  
Jia-Li Liao ◽  
Albert J.C. Chen ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
High Performance ◽  
Free Zone ◽  
Carrier Free ◽  
Zone Electrophoresis

Free zone electrophoresis

1967 ◽  
Vol 9 (2) ◽  
pp. 122-219 ◽  
Author(s):  
Stellan Hjertén
Keyword(s):  
Free Zone ◽  
Zone Electrophoresis

Role of charge suppression and ionic strength in free zone electrophoresis of proteins

1991 ◽  
Vol 63 (22) ◽  
pp. 2597-2602 ◽  
Author(s):  
Bruce Jon. Compton ◽  
Elizabeth A. O'Grady
Keyword(s):  
Ionic Strength ◽  
Free Zone ◽  
Zone Electrophoresis ◽  
Electrophoresis Of Proteins

Concentration distributions in free zone electrophoresis

1979 ◽  
Vol 169 ◽  
pp. 1-10 ◽  
Author(s):  
F.E.P. Mikkers ◽  
F.M. Everaerts ◽  
Th.P.E.M. Verheggen
Keyword(s):  
Free Zone ◽  
Zone Electrophoresis
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