scholarly journals A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization

2016 ◽  
pp. 77-107
Author(s):  
Stephen R. Okoniewski ◽  
Ashley R. Carter ◽  
Thomas T. Perkins
Keyword(s):  
Optical Trap ◽  
Active Stabilization

scholarly journals Biomechanics of Neutrophil Tethers

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 515
Author(s):  
Andrea Cugno ◽  
Alex Marki ◽  
Klaus Ley
Keyword(s):  
Cell Activation ◽  
Optical Trap ◽  
Biomechanical Properties ◽  
Force Microscopy ◽  
Advantages And Disadvantages ◽  
Atomic Force ◽  
Microfluidic Flow ◽  
Biomembrane Force Probe ◽  
Membrane Reservoir

Leukocytes, including neutrophils, which are propelled by blood flow, can roll on inflamed endothelium using transient bonds between selectins and their ligands, and integrins and their ligands. When such receptor–ligand bonds last long enough, the leukocyte microvilli become extended and eventually form thin, 20 m long tethers. Tether formation can be observed in blood vessels in vivo and in microfluidic flow chambers. Tethers can also be extracted using micropipette aspiration, biomembrane force probe, optical trap, or atomic force microscopy approaches. Here, we review the biomechanical properties of leukocyte tethers as gleaned from such measurements and discuss the advantages and disadvantages of each approach. We also review and discuss viscoelastic models that describe the dependence of tether formation on time, force, rate of loading, and cell activation. We close by emphasizing the need to combine experimental observations with quantitative models and computer simulations to understand how tether formation is affected by membrane tension, membrane reservoir, and interactions of the membrane with the cytoskeleton.

scholarly journals Compact ultracold electron source based on a grating magneto-optical trap

2019 ◽  
Vol 22 (2) ◽  
Author(s):  
J. G. H. Franssen ◽  
T. C. H. de Raadt ◽  
M. A. W. van Ninhuijs ◽  
O. J. Luiten
Keyword(s):  
Optical Trap ◽  
Electron Source ◽  
Magneto Optical Trap

scholarly journals Narrow-line magneto-optical trap for europium

2021 ◽  
Vol 103 (5) ◽  
Author(s):  
Yuki Miyazawa ◽  
Ryotaro Inoue ◽  
Hiroki Matsui ◽  
Kenta Takanashi ◽  
Mikio Kozuma
Keyword(s):  
Optical Trap ◽  
Magneto Optical Trap ◽  
Narrow Line

scholarly journals Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events

Micromachines ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 570
Author(s):  
Alexander B. Stilgoe ◽  
Declan J. Armstrong ◽  
Halina Rubinsztein-Dunlop
Keyword(s):  
Brownian Motion ◽  
Optical Tweezers ◽  
Optical Trap ◽  
Force Transducer ◽  
Signal To Noise ◽  
Likelihood Estimator ◽  
Micro Fluidics ◽  
Large Numbers ◽  
Force Reconstruction ◽  
Micron Particle

The trap stiffness us the key property in using optical tweezers as a force transducer. Force reconstruction via maximum-likelihood-estimator analysis (FORMA) determines the optical trap stiffness based on estimation of the particle velocity from statistical trajectories. Using a modification of this technique, we determine the trap stiffness for a two micron particle within 2 ms to a precision of ∼10% using camera measurements at 10 kfps with the contribution of pixel noise to the signal being larger the level Brownian motion. This is done by observing a particle fall into an optical trap once at a high stiffness. This type of calibration is attractive, as it avoids the use of a nanopositioning stage, which makes it ideal for systems of large numbers of particles, e.g., micro-fluidics or active matter systems.

Improved trapping in a vapor-cell magneto-optical trap with multiple laser frequencies

1994 ◽  
Vol 11 (12) ◽  
pp. 2333 ◽  
Author(s):  
Alastair G. Sinclair ◽  
Erling Riis ◽  
Michael J. Snadden
Keyword(s):  
Optical Trap ◽  
Magneto Optical Trap ◽  
Vapor Cell ◽  
Laser Frequencies

Publisher's Note: Evidence of sympathetic cooling of Na+ions by a Na magneto-optical trap in a hybrid trap [Phys. Rev. A86, 063419 (2012)]

2013 ◽  
Vol 87 (1) ◽  
Author(s):  
I. Sivarajah ◽  
D. S. Goodman ◽  
J. E. Wells ◽  
F. A. Narducci ◽  
W. W. Smith
Keyword(s):  
Optical Trap ◽  
Magneto Optical Trap ◽  
Sympathetic Cooling ◽  
Na Ions

ORIENTATING MANIPULATION OF CYLINDRICAL PARTICLES WITH OPTICAL TWEEZERS

2008 ◽  
Vol 17 (04) ◽  
pp. 387-394 ◽  
Author(s):  
XIUDONG SUN ◽  
XUECONG LI ◽  
JIANLONG ZHANG
Keyword(s):  
Escherichia Coli ◽  
Carbon Nanotubes ◽  
Laser Beam ◽  
Optical Tweezers ◽  
Optical Trap ◽  
Polarization Direction ◽  
Beam Shape ◽  
E Coli ◽  
Potential Applications ◽  
Cylindrical Particles

Orientating manipulations of cylindrical particles were performed by optical tweezers. Vertical and horizontal manipulations of Escherichia coli (E. coli) were carried out by changing the trapping depth and the focused laser beam shape. It was found that carbon nanotubes bundles (CNTBs) could be rotated in the linear polarized optical trap until it orientated its long axis along the linear polarization direction of the laser beam. However, E.coli could not be orientated in this way. Corresponding mechanisms were discussed based on the anisomeric electric characters of CNTBs. These orientation technologies of cylindrical objects with optical trap have potential applications in assembling nano-electric devices.

Adaptive disturbance rejection in an optical trap

2008 ◽  
Vol 47 (20) ◽  
pp. 3585 ◽  
Author(s):  
Kurt D. Wulff ◽  
Daniel G. Cole ◽  
Robert L. Clark
Keyword(s):  
Disturbance Rejection ◽  
Optical Trap
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