Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems

2016 ◽  
pp. 1-15 ◽  
Author(s):  
Jacqueline Krüger ◽  
Dorit Schleinitz
Keyword(s):  
Microsatellite Markers ◽  
Multiplex Pcr ◽  
Primer Design ◽  
Genetic Fingerprinting ◽  
Detection Systems ◽  
Methodological Approaches

scholarly journals Primer Design for the Identification of Ten Oral Actinomyces Species Using Multiplex PCR

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Kobayashi T ◽  
Uchibori S ◽  
Tsuzukibashi O ◽  
Uezato C ◽  
Goto H ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Primer Design ◽  
Actinomyces Species

Characterization of 33 microsatellite markers and development of multiplex PCR for yellow-throated marten (Martes flavigula)

2017 ◽  
Vol 16 (4) ◽  
Author(s):  
Mu Yeong Lee ◽  
Hea Chang Moon ◽  
Hey Sook Jeon ◽  
Eui Geun Song ◽  
Donggul Woo ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Multiplex Pcr

Individual Identification using The Multiplex PCR with Microsatellite Markers in Swine

2013 ◽  
Vol 37 (4) ◽  
pp. 205-211
Author(s):  
Lee-Kung Kima ◽  
◽  
Chang-Min Parka ◽  
Sun-Ae Park ◽  
Seung-Chang Kim ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Multiplex Pcr ◽  
Individual Identification

scholarly journals Group-Specific Multiplex PCR Detection Systems for the Identification of Flying Insect Prey

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e115501 ◽  
Author(s):  
Daniela Sint ◽  
Bettina Niederklapfer ◽  
Ruediger Kaufmann ◽  
Michael Traugott
Keyword(s):  
Multiplex Pcr ◽  
Pcr Detection ◽  
Insect Prey ◽  
Detection Systems

scholarly journals Single-Tube Dodecaplex PCR Panel of Polymorphic Microsatellite Markers Closely Linked to the DMPK CTG Repeat for Preimplantation Genetic Diagnosis of Myotonic Dystrophy Type 1

2017 ◽  
Vol 63 (6) ◽  
pp. 1127-1140 ◽  
Author(s):  
Mulias Lian ◽  
Mingjue Zhao ◽  
Caroline G Lee ◽  
Samuel S Chong
Keyword(s):  
Microsatellite Markers ◽  
Multiplex Pcr ◽  
Polymorphism Information Content ◽  
Single Cells ◽  
Genetic Diagnosis ◽  
Myotonic Dystrophy Type 1 ◽  
Myotonic Dystrophy Type ◽  
Single Tube ◽  
Ctg Repeat

Abstract BACKGROUND Preimplantation genetic diagnosis (PGD) of myotonic dystrophy type 1 (DM1) currently uses conventional PCR to detect nonexpanded dystrophia myotonica protein kinase (DMPK) alleles or triplet-primed PCR to detect the CTG-expanded alleles, coupled with analysis of linked microsatellite markers to increase diagnostic accuracy. We aimed to simplify the process of identification and selection of informative linked markers for application to DM1 PGD. METHODS An in silico search was performed to identify all markers within 1–1.5 Mb flanking the DMPK gene. Five previously known (D19S559, APOC2, D19S543, D19S112, and BV209569) and 7 novel (DM45050, DM45178, DM45209, DM45958, DM46513, DM46892, and DM47004.1) markers with potentially high heterozygosity values and polymorphism information content were selected and optimized in a single-tube multiplex PCR panel. RESULTS Analysis of 184 DNA samples of Chinese and Caucasian individuals (91 from unrelated, anonymized cord blood of Chinese babies born at the National University Hospital, Singapore, and 93 Caucasian DNA samples from the Human Variation Panel HD100CAU) confirmed the high polymorphism indices of all markers (polymorphism information content >0.5), with observed heterozygosity values ranging from 0.62–0.93. All individuals were heterozygous for at least 6 markers, with 99.5% of individuals heterozygous for at least 2 markers on either side of the DMPK CTG repeat. The dodecaplex marker assay was successfully validated on 42 single cells and 12 whole genome amplified single cells. CONCLUSIONS The DM1 multiplex PCR panel is suitable for use in DM1 PGD either as a standalone linkage-based assay or as a complement to DMPK CTG repeat expansion-mutation detection.

Primer design for identifying economically important L iriomyza species ( D iptera: A gromyzidae) by multiplex PCR

2012 ◽  
Vol 13 (1) ◽  
pp. 96-102 ◽  
Author(s):  
Shigeo Nakamura ◽  
Toshio Masuda ◽  
Atsushi Mochizuki ◽  
Kazuhiko Konishi ◽  
Susumu Tokumaru ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Primer Design
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