The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair

Methods in Molecular Biology - Cohesin and Condensin ◽

10.1007/978-1-4939-6545-8_14 ◽

2016 ◽

pp. 227-242 ◽

Cited By ~ 2

Author(s):

Xiangduo Kong ◽

Alexander R. Ball ◽

Kyoko Yokomori

Keyword(s):

Dna Repair ◽

Laser Microirradiation

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The histone H3 variant CENP‐A localizes to sites of DNA repair induced by laser microirradiation of living cells

The FASEB Journal ◽

10.1096/fasebj.22.1_supplement.600.3 ◽

2008 ◽

Vol 22 (S1) ◽

Author(s):

Sam G. Zeitlin ◽

Norman M Baker ◽

Jean JY Wang ◽

Michael W Berns ◽

Don W Cleveland

Keyword(s):

Dna Repair ◽

Histone H3 ◽

Living Cells ◽

Histone H3 Variant ◽

Laser Microirradiation

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Q-FADD: A mechanistic approach for modeling the accumulation of proteins at sites of DNA damage by free diffusion

10.1101/373043 ◽

2018 ◽

Cited By ~ 2

Author(s):

Jyothi Mahadevan ◽

Johannes Rudolph ◽

Asmita Jha ◽

Jian Wei Tay ◽

Joe Dragavon ◽

...

Keyword(s):

Dna Damage ◽

Dna Repair ◽

Diffusion Model ◽

Live Cells ◽

Free Diffusion ◽

Mechanistic Approach ◽

Laser Microirradiation ◽

Rigorous Method

AbstractThe repair of DNA damage requires the ordered recruitment of many different proteins that are responsible for signaling and subsequent repair. A powerful tool for studying the orchestrated accumulation of these proteins at damage sites is laser microirradiation in live cells, followed by monitoring of the accumulation of the fluorescently labeled protein in question. Despite the widespread use of this approach, there exists no rigorous method for characterizing this process quantitatively. Here we introduce a free diffusion model that explicitly accounts for the unique topology of individual nuclei and quantitatively describes the accumulation of two test proteins, poly-ADP-ribose polymerases 1 and 2. Application of our model to other proteins will yield novel insights into the timing and mechanism of DNA repair.

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