Micropatterned Co-Cultures of Induced Pluripotent Stem Cell-Derived Hepatocytes and Stromal Cells for Drug Toxicity Studies

2016 ◽  
pp. 311-334
Author(s):  
Brenton R. Ware ◽  
Salman R. Khetani
Keyword(s):  
Stem Cell ◽  
Stromal Cells ◽  
Drug Toxicity ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Toxicity Studies ◽  
Induced Pluripotent

The development of induced pluripotent stem cell-derived mesenchymal stem/stromal cells from normal human and RDEB epidermal keratinocytes

2018 ◽  
Vol 91 (3) ◽  
pp. 301-310 ◽  
Author(s):  
Chihiro Nakayama ◽  
Yasuyuki Fujita ◽  
Wakana Matsumura ◽  
Inkin Ujiie ◽  
Shota Takashima ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stromal Cells ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Epidermal Keratinocytes ◽  
Normal Human ◽  
Induced Pluripotent

scholarly journals Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells

2019 ◽  
Vol 37 ◽  
pp. 101409
Author(s):  
Timur Bikkuzin ◽  
Yan Shi ◽  
Baoqi Sun ◽  
Yiyuan Guo ◽  
Xin Jin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Stromal Cells ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Stem Cell Line ◽  
Induced Pluripotent

scholarly journals Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes, in Contrast to Adipose Tissue-Derived Stromal Cells, Efficiently Improve Heart Function in Murine Model of Myocardial Infarction

Biomedicines ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 578
Author(s):  
Jacek Stępniewski ◽  
Mateusz Tomczyk ◽  
Kalina Andrysiak ◽  
Izabela Kraszewska ◽  
Alicja Martyniak ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cell ◽  
Therapeutic Effect ◽  
Murine Model ◽  
Stromal Cells ◽  
Pluripotent Stem Cell ◽  
Heart Function ◽  
Genetically Modified ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

Cell therapies are extensively tested to restore heart function after myocardial infarction (MI). Survival of any cell type after intracardiac administration, however, may be limited due to unfavorable conditions of damaged tissue. Therefore, the aim of this study was to evaluate the therapeutic effect of adipose-derived stromal cells (ADSCs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) overexpressing either the proangiogenic SDF-1α or anti-inflammatory heme oxygenase-1 (HO-1) in a murine model of MI. ADSCs and hiPSCs were transduced with lentiviral vectors encoding luciferase (Luc), GFP and either HO-1 or SDF-1α. hiPSCs were then differentiated to hiPSC-CMs using small molecules modulating the WNT pathway. Genetically modified ADSCs were firstly administered via intracardiac injection after MI induction in Nude mice. Next, ADSCs-Luc-GFP and genetically modified hiPSC-CMs were injected into the hearts of the more receptive NOD/SCID strain to compare the therapeutic effect of both cell types. Ultrasonography, performed on days 7, 14, 28 and 42, revealed a significant decrease of left ventricular ejection fraction (LVEF) in all MI-induced groups. No improvement of LVEF was observed in ADSC-treated Nude and NOD/SCID mice. In contrast, administration of hiPSC-CMs resulted in a substantial increase of LVEF, occurring between 28 and 42 days after MI, and decreased fibrosis, regardless of genetic modification. Importantly, bioluminescence analysis, as well as immunofluorescent staining, confirmed the presence of hiPSC-CMs in murine tissue. Interestingly, the luminescence signal was strongest in hearts treated with hiPSC-CMs overexpressing HO-1. Performed experiments demonstrate that hiPSC-CMs, unlike ADSCs, are effective in improving heart function after MI. Additionally, long-term evaluation of heart function seems to be crucial for proper assessment of the effect of cell administration.

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