Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice

Faculty Opinions recommendation of Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717958108.793462412 ◽

2012 ◽

Author(s):

Alisdair Fernie ◽

Wagner Araujo

Keyword(s):

Drought Stress ◽

Brachypodium Distachyon ◽

Physiological Analysis

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PHYSIOLOGICAL ANALYSIS OF THE GROWTH AND PRODUCTIVITY OF RADISH VARIÅTIES

Agricultural sciences ◽

10.22620/agrisci.2009.01.008 ◽

2009 ◽

Vol 1 (1) ◽

pp. 53-58

Author(s):

Walid al Humrani

Keyword(s):

Physiological Analysis

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Physiological and interactomic analysis reveals versatile functions of Arabidopsis 14-3-3 quadruple mutants in response to Fe deficiency

Scientific Reports ◽

10.1038/s41598-021-94908-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jing Gao ◽

Paula J. M. van Kleeff ◽

Ka Wan Li ◽

Albertus H. de Boer

Keyword(s):

Iron Acquisition ◽

Tca Cycle ◽

Utilization Efficiency ◽

Fe Deficiency ◽

Transcriptional Level ◽

Functional Aspects ◽

Clear Explanation ◽

Physiological Analysis ◽

Proper Operation

AbstractTo date, few phenotypes have been described for Arabidopsis 14-3-3 mutants or the phenotypes showing the role of 14-3-3 in plant responding to abiotic stress. Although one member of the 14-3-3 protein family (14-3-3 omicron) was shown to be involved in the proper operation of Fe acquisition mechanisms at physiological and gene expression levels in Arabidopsis thaliana, it remains to be explored whether other members play a role in regulating iron acquisition. To more directly and effectively observe whether members of 14-3-3 non-epsilon group have a function in Fe-deficiency adaptation, three higher order quadruple KOs, kappa/lambda/phi/chi (klpc), kappa/lambda/upsilon/nu(klun), and upsilon/nu/phi/chi (unpc) were generated and studied for physiological analysis in this study. The analysis of iron-utilization efficiency, root phenotyping, and transcriptional level of Fe-responsive genes suggested that the mutant with kl background showed different phenotypes from Wt when plants suffered Fe starved, while these phenotypes were absent in the unpc mutant. Moreover, the absence of the four 14-3-3 isoforms in the klun mutant has a clear impact on the 14-3-3 interactome upon Fe deficiency. Dynamics of 14-3-3-client interactions analysis showed that 27 and 17 proteins differentially interacted with 14-3-3 in Wt and klun roots caused by Fe deficiency, respectively. Many of these Fe responsive proteins have a role in glycolysis, oxidative phosphorylation and TCA cycle, the FoF1-synthase and in the cysteine/methionine synthesis. A clear explanation for the observed phenotypes awaits a more detailed analysis of the functional aspects of 14-3-3 binding to the target proteins identified in this study.

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Comparative transcriptomic and physiological analyses of weedy rice and cultivated rice to identify vital differentially expressed genes and pathways regulating the ABA response

Scientific Reports ◽

10.1038/s41598-021-92504-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Hong Lang ◽

Yuting He ◽

Faliang Zeng ◽

Fan Xu ◽

Minghui Zhao ◽

...

Keyword(s):

Differentially Expressed Genes ◽

Differentially Expressed ◽

Weedy Rice ◽

Cultivated Rice ◽

Biotic Stresses ◽

Aba Sensitivity ◽

Aba Catabolism ◽

Physiological Analysis ◽

Response To Stress ◽

Aba Response

AbstractWeedy rice is a valuable germplasm resource characterized by its high tolerance to both abiotic and biotic stresses. Abscisic acid (ABA) serves as a regulatory signal in plant cells as part of their adaptive response to stress. However, a global understanding of the response of weedy rice to ABA remains to be elucidated. In the present study, the sensitivity to ABA of weedy rice (WR04-6) was compared with that of temperate japonica Shennong9816 (SN9816) in terms of seed germination and post-germination growth via the application of exogenous ABA and diniconazole, an inhibitor of ABA catabolism. Physiological analysis and a transcriptomic comparison allowed elucidation of the molecular and physiological mechanisms associated with continuous ABA and diniconazole treatment. WR04-6 was found to display higher ABA sensitivity than SN9816, resulting in the rapid promotion of antioxidant enzyme activity. Comparative transcriptomic analyses indicated that the number of differentially expressed genes (DEGs) in WR04-6 seedlings treated with 2 μM ABA or 10 μM diniconazole was greater than that in SN9816 seedlings. Genes involved in stress defense, hormone signal transduction, and glycolytic and citrate cycle pathways were highly expressed in WR04-6 in response to ABA and diniconazole. These findings provide new insight into key processes mediating the ABA response between weedy and cultivated rice.

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Kiss1 Neurons Drastically Change Their Firing Activity in Accordance With the Reproductive State: Insights From a Seasonal Breeder

Endocrinology ◽

10.1210/en.2014-1472 ◽

2014 ◽

Vol 155 (12) ◽

pp. 4868-4880 ◽

Cited By ~ 8

Author(s):

Masaharu Hasebe ◽

Shinji Kanda ◽

Hiroyuki Shimada ◽

Yasuhisa Akazome ◽

Hideki Abe ◽

...

Keyword(s):

Fluorescent Protein ◽

Resting Membrane Potential ◽

Reproductive State ◽

Firing Patterns ◽

State Dependent ◽

Channel Expression ◽

Physiological Analysis ◽

Seasonal Breeder ◽

Green Fluorescent

Kisspeptin (Kiss) neurons show drastic changes in kisspeptin expression in response to the serum sex steroid concentration in various vertebrate species. Thus, according to the reproductive states, kisspeptin neurons are suggested to modulate various neuronal activities, including the regulation of GnRH neurons in mammals. However, despite their reproductive state-dependent regulation, there is no physiological analysis of kisspeptin neurons in seasonal breeders. Here we generated the first kiss1-enhanced green fluorescent protein transgenic line of a seasonal breeder, medaka, for histological and electrophysiological analyses using a whole-brain in vitro preparation in which most synaptic connections are intact. We found histologically that Kiss1 neurons in the nucleus ventralis tuberis (NVT) projected to the preoptic area, hypothalamus, pituitary, and ventral telencephalon. Therefore, NVT Kiss1 neurons may regulate various homeostatic functions and innate behaviors. Electrophysiological analyses revealed that they show various firing patterns, including bursting. Furthermore, we found that their firings are regulated by the resting membrane potential. However, bursting was not induced from the other firing patterns with a current injection, suggesting that it requires some chronic modulations of intrinsic properties such as channel expression. Finally, we found that NVT Kiss1 neurons drastically change their neuronal activities according to the reproductive state and the estradiol levels. Taken together with the previous reports, we here conclude that the breeding condition drastically alters the Kiss1 neuron activities in both gene expression and firing activities, the latter of which is strongly related to Kiss1 release, and the Kiss1 peptides regulate the activities of various neural circuits through their axonal projections.

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