Circular Chromosome Conformation Capture in Plants

2017 ◽  
pp. 73-92 ◽  
Author(s):  
Stefan Grob
Keyword(s):  
Chromosome Conformation Capture ◽  
Circular Chromosome ◽  
Chromosome Conformation ◽  
Circular Chromosome Conformation Capture

scholarly journals Male-Specific Long Noncoding RNA TTTY15 Inhibits Non-Small Cell Lung Cancer Proliferation and Metastasis via TBX4

2019 ◽  
Vol 20 (14) ◽  
pp. 3473 ◽  
Author(s):  
I-Lu Lai ◽  
Ya-Sian Chang ◽  
Wen-Ling Chan ◽  
Ya-Ting Lee ◽  
Ju-Chen Yen ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Chromosome Conformation Capture ◽  
Small Cell Lung ◽  
Circular Chromosome ◽  
Chromosome Conformation ◽  
Male Specific ◽  
Circular Chromosome Conformation Capture

Gender affects cancer susceptibility. Currently, there are only a few studies on Y chromosome-linked long noncoding RNAs (lncRNAs), and the potential association between lncRNAs and cancers in males has not been fully elucidated. Here, we examined the expression of testis-specific transcript Y-linked 15 (TTTY15) in 37 males with non-small cell lung cancer (NSCLC), and performed circular chromosome conformation capture with next-generation sequencing to determine the genomic interaction regions of the TTTY15 gene. Our results showed that the expression levels of TTTY15 were lower in NSCLC tissues. Lower TTTY15 expression levels were associated with Tumor-Node-Metastasis (TNM) stage. A TTTY15 knockdown promoted malignant transformation of NSCLC cells. Based on the bioinformatics analysis of circular chromosome conformation capture data, we found that T-box transcription factor 4 (TBX4) may be a potential target gene of TTTY15. The RNA immunoprecipitation and chromatin immunoprecipitation results showed that TTTY15 may interact with DNA (cytosine-5)-methyltransferase 3A (DNMT3A), and the TTTY15 knockdown increased the binding of DNMT3A to the TBX4 promoter. We concluded that low TTTY15 expression correlates with worse prognosis among patients with NSCLC. TTTY15 promotes TBX4 expression via DNMT3A-mediated regulation. The identification of lncRNAs encoded by male-specific genes may help to identify potential targets for NSCLC therapy.

Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactions

2006 ◽  
Vol 38 (11) ◽  
pp. 1341-1347 ◽  
Author(s):  
Zhihu Zhao ◽  
Gholamreza Tavoosidana ◽  
Mikael Sjölinder ◽  
Anita Göndör ◽  
Piero Mariano ◽  
...  
Keyword(s):  
Chromosome Conformation Capture ◽  
Circular Chromosome ◽  
Chromosome Conformation ◽  
Circular Chromosome Conformation Capture
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