The A-Like Faker Assay for Measuring Yeast Chromosome III Stability

Author(s):  
Carolina A. Novoa ◽  
J. Sidney Ang ◽  
Peter C. Stirling

1988 ◽  
Vol 8 (2) ◽  
pp. 595-604
Author(s):  
L S Symington ◽  
T D Petes

To examine the relationship between genetic and physical chromosome maps, we constructed a diploid strain of the yeast Saccharomyces cerevisiae heterozygous for 12 restriction site mutations within a 23-kilobase (5-centimorgan) interval of chromosome III. Crossovers were not uniformly distributed along the chromosome, one interval containing significantly more and one interval significantly fewer crossovers than expected. One-third of these crossovers occurred within 6 kilobases of the centromere. Approximately half of the exchanges were associated with gene conversion events. The minimum length of gene conversion tracts varied from 4 base pairs to more than 12 kilobases, and these tracts were nonuniformly distributed along the chromosome. We conclude that the chromosomal sequence or structure has a dramatic effect on meiotic recombination.





Yeast ◽  
1992 ◽  
Vol 8 (9) ◽  
pp. 805-812 ◽  
Author(s):  
M. L. Agostoni Carbone ◽  
L. Panzeri ◽  
M. Muzi Falconi ◽  
C. Carcano ◽  
P. Plevani ◽  
...  


1992 ◽  
Vol 12 (10) ◽  
pp. 4733-4741 ◽  
Author(s):  
J Zhu ◽  
C S Newlon ◽  
J A Huberman

Two-dimensional gel electrophoretic replicon mapping techniques were used to identify all functional DNA replication origins and termini in a 26.5-kbp stretch in the left arm of yeast chromosome III. Only one origin was detected; it coincided with an ARS element (ARS306), as have all previously mapped yeast origins. A replication termination region was identified in a 4.3-kbp stretch at the telomere-proximal end of the investigated region, between the origin identified in this paper and the neighboring, previously mapped, ARS305-associated origin (previously called the A6C origin). Termination does not occur at a specific site; instead, it appears to be the consequence of replication forks converging in a stretch of DNA of at least 4.3 kbp.



1988 ◽  
Vol 8 (2) ◽  
pp. 595-604 ◽  
Author(s):  
L S Symington ◽  
T D Petes

To examine the relationship between genetic and physical chromosome maps, we constructed a diploid strain of the yeast Saccharomyces cerevisiae heterozygous for 12 restriction site mutations within a 23-kilobase (5-centimorgan) interval of chromosome III. Crossovers were not uniformly distributed along the chromosome, one interval containing significantly more and one interval significantly fewer crossovers than expected. One-third of these crossovers occurred within 6 kilobases of the centromere. Approximately half of the exchanges were associated with gene conversion events. The minimum length of gene conversion tracts varied from 4 base pairs to more than 12 kilobases, and these tracts were nonuniformly distributed along the chromosome. We conclude that the chromosomal sequence or structure has a dramatic effect on meiotic recombination.



1992 ◽  
Vol 1 (12) ◽  
pp. 1677-1690 ◽  
Author(s):  
Peer Bork ◽  
Christos Ouzounis ◽  
Chris Sander ◽  
Michael Scharf ◽  
Reinhard Schneider ◽  
...  


Science ◽  
1992 ◽  
Vol 256 (5058) ◽  
pp. 730-730 ◽  
Author(s):  
F Eijgenraam


1992 ◽  
Vol 10 (7) ◽  
pp. 761-761


Cell ◽  
1979 ◽  
Vol 18 (2) ◽  
pp. 309-319 ◽  
Author(s):  
Jeffrey N. Strathern ◽  
Carol S. Newlon ◽  
Ira Herskowitz ◽  
James B. Hicks


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