The frequency of oligopurine. Oligopyrimidine and other two-base tracts in yeast chromosome III

Yeast ◽  
1994 ◽  
Vol 10 (5) ◽  
pp. 603-611 ◽  
Author(s):  
G. Yagil

1988 ◽  
Vol 8 (2) ◽  
pp. 595-604
Author(s):  
L S Symington ◽  
T D Petes

To examine the relationship between genetic and physical chromosome maps, we constructed a diploid strain of the yeast Saccharomyces cerevisiae heterozygous for 12 restriction site mutations within a 23-kilobase (5-centimorgan) interval of chromosome III. Crossovers were not uniformly distributed along the chromosome, one interval containing significantly more and one interval significantly fewer crossovers than expected. One-third of these crossovers occurred within 6 kilobases of the centromere. Approximately half of the exchanges were associated with gene conversion events. The minimum length of gene conversion tracts varied from 4 base pairs to more than 12 kilobases, and these tracts were nonuniformly distributed along the chromosome. We conclude that the chromosomal sequence or structure has a dramatic effect on meiotic recombination.



Yeast ◽  
1992 ◽  
Vol 8 (9) ◽  
pp. 805-812 ◽  
Author(s):  
M. L. Agostoni Carbone ◽  
L. Panzeri ◽  
M. Muzi Falconi ◽  
C. Carcano ◽  
P. Plevani ◽  
...  


1992 ◽  
Vol 12 (10) ◽  
pp. 4733-4741 ◽  
Author(s):  
J Zhu ◽  
C S Newlon ◽  
J A Huberman

Two-dimensional gel electrophoretic replicon mapping techniques were used to identify all functional DNA replication origins and termini in a 26.5-kbp stretch in the left arm of yeast chromosome III. Only one origin was detected; it coincided with an ARS element (ARS306), as have all previously mapped yeast origins. A replication termination region was identified in a 4.3-kbp stretch at the telomere-proximal end of the investigated region, between the origin identified in this paper and the neighboring, previously mapped, ARS305-associated origin (previously called the A6C origin). Termination does not occur at a specific site; instead, it appears to be the consequence of replication forks converging in a stretch of DNA of at least 4.3 kbp.



1988 ◽  
Vol 8 (2) ◽  
pp. 595-604 ◽  
Author(s):  
L S Symington ◽  
T D Petes

To examine the relationship between genetic and physical chromosome maps, we constructed a diploid strain of the yeast Saccharomyces cerevisiae heterozygous for 12 restriction site mutations within a 23-kilobase (5-centimorgan) interval of chromosome III. Crossovers were not uniformly distributed along the chromosome, one interval containing significantly more and one interval significantly fewer crossovers than expected. One-third of these crossovers occurred within 6 kilobases of the centromere. Approximately half of the exchanges were associated with gene conversion events. The minimum length of gene conversion tracts varied from 4 base pairs to more than 12 kilobases, and these tracts were nonuniformly distributed along the chromosome. We conclude that the chromosomal sequence or structure has a dramatic effect on meiotic recombination.



1992 ◽  
Vol 1 (12) ◽  
pp. 1677-1690 ◽  
Author(s):  
Peer Bork ◽  
Christos Ouzounis ◽  
Chris Sander ◽  
Michael Scharf ◽  
Reinhard Schneider ◽  
...  


Science ◽  
1992 ◽  
Vol 256 (5058) ◽  
pp. 730-730 ◽  
Author(s):  
F Eijgenraam


1992 ◽  
Vol 10 (7) ◽  
pp. 761-761


Cell ◽  
1979 ◽  
Vol 18 (2) ◽  
pp. 309-319 ◽  
Author(s):  
Jeffrey N. Strathern ◽  
Carol S. Newlon ◽  
Ira Herskowitz ◽  
James B. Hicks


1984 ◽  
Vol 99 (5) ◽  
pp. 1559-1568 ◽  
Author(s):  
K S Bloom ◽  
E Amaya ◽  
J Carbon ◽  
L Clarke ◽  
A Hill ◽  
...  

The centromere region of Saccharomyces cerevisiae chromosome III has been replaced by various DNA fragments from the centromere regions of yeast chromosomes III and XI. A 289-base pair centromere (CEN3) sequence can stabilize yeast chromosome III through mitosis and meiosis. The orientation of the centromeric fragments within chromosome III has no effect on the normal mitotic or meiotic behavior of the chromosome. The structural integrity of the centromere region in these genomic substitution strains was examined by mapping nucleolytic cleavage sites within the chromatin DNA. A nuclease-protected centromere core of 220-250 base pairs was evident in all of the genomic substitution strains. The position of the protected region is determined strictly by the centromere DNA sequence. These results indicate that the functional centromere core is contained within 220-250 base pairs of the chromatin DNA that is structurally distinct from the flanking nucleosomal chromatin.



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