scholarly journals Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy

2018 ◽  
pp. 45-58 ◽  
Author(s):  
Brian L. Gilmore ◽  
A. Cameron Varano ◽  
William Dearnaley ◽  
Yanping Liang ◽  
Bridget C. Marcinkowski ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Protein Complexes ◽  
Native Protein ◽  
Single Particle Electron Microscopy

Modular Imaging Scaffold for Single-Particle Electron Microscopy

ACS Nano ◽  
2021 ◽  
Author(s):  
Nesrine Aissaoui ◽  
Josephine Lai-Kee-Him ◽  
Allan Mills ◽  
Nathalie Declerck ◽  
Zakia Morichaud ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy

scholarly journals Electron microscopy snapshots of single particles from single cells

2018 ◽  
Vol 294 (5) ◽  
pp. 1602-1608 ◽  
Author(s):  
Xiunan Yi ◽  
Eric J. Verbeke ◽  
Yiran Chang ◽  
Daniel J. Dickinson ◽  
David W. Taylor
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Protein Complexes ◽  
Signal To Noise Ratio ◽  
Single Cells ◽  
Particle Analysis ◽  
Biological Macromolecules ◽  
Single Particle Analysis ◽  
Whole Cells ◽  
Potential Applications

Cryo-electron microscopy (cryo-EM) has become an indispensable tool for structural studies of biological macromolecules. Two additional predominant methods are available for studying the architectures of multiprotein complexes: 1) single-particle analysis of purified samples and 2) tomography of whole cells or cell sections. The former can produce high-resolution structures but is limited to highly purified samples, whereas the latter can capture proteins in their native state but has a low signal-to-noise ratio and yields lower-resolution structures. Here, we present a simple, adaptable method combining microfluidic single-cell extraction with single-particle analysis by EM to characterize protein complexes from individual Caenorhabditis elegans embryos. Using this approach, we uncover 3D structures of ribosomes directly from single embryo extracts. Moreover, we investigated structural dynamics during development by counting the number of ribosomes per polysome in early and late embryos. This approach has significant potential applications for counting protein complexes and studying protein architectures from single cells in developmental, evolutionary, and disease contexts.

scholarly journals Deblurring of class-averaged images in single-particle electron microscopy

2010 ◽  
Vol 26 (3) ◽  
pp. 035002 ◽  
Author(s):  
Wooram Park ◽  
Dean R Madden ◽  
Daniel N Rockmore ◽  
Gregory S Chirikjian
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy
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