Fast Small-Scale Membrane Protein Purification and Grid Preparation for Single-Particle Electron Microscopy

2020 ◽  
pp. 275-282
Author(s):  
Natalie Bärland ◽  
Camilo Perez
Keyword(s):  
Electron Microscopy ◽  
Membrane Protein ◽  
Protein Purification ◽  
Single Particle ◽  
Small Scale ◽  
Single Particle Electron Microscopy

scholarly journals Structure and assembly of CAV1 8S complexes revealed by single particle electron microscopy

2020 ◽  
Vol 6 (49) ◽  
pp. eabc6185
Author(s):  
Bing Han ◽  
Jason C. Porta ◽  
Jessica L. Hanks ◽  
Yelena Peskova ◽  
Elad Binshtein ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Membrane Protein ◽  
Single Particle ◽  
Structural Organization ◽  
Building Blocks ◽  
Structural Features ◽  
Outer Ring ◽  
Current Evidence ◽  
Caveolin 1 ◽  
Single Particle Electron Microscopy

Highly stable oligomeric complexes of the monotopic membrane protein caveolin serve as fundamental building blocks of caveolae. Current evidence suggests these complexes are disc shaped, but the details of their structural organization and how they assemble are poorly understood. Here, we address these questions using single particle electron microscopy of negatively stained recombinant 8S complexes of human caveolin 1. We show that 8S complexes are toroidal structures ~15 nm in diameter that consist of an outer ring, an inner ring, and central protruding stalk. Moreover, we map the position of the N and C termini and determine their role in complex assembly, and visualize the 8S complexes in heterologous caveolae. Our findings provide critical insights into the structural features of 8S complexes and allow us to propose a model for how these highly stable membrane-embedded complexes are generated.

scholarly journals Single-particle electron microscopy in the study of membrane protein structure

Microscopy ◽  
2015 ◽  
Vol 65 (1) ◽  
pp. 81-96 ◽  
Author(s):  
Rita De Zorzi ◽  
Wei Mi ◽  
Maofu Liao ◽  
Thomas Walz
Keyword(s):  
Electron Microscopy ◽  
Protein Structure ◽  
Membrane Protein ◽  
Single Particle ◽  
Membrane Protein Structure ◽  
Single Particle Electron Microscopy

scholarly journals Structure and assembly of CAV1 8S complexes revealed by single particle electron microscopy

2020 ◽  
Author(s):  
Bing Han ◽  
Jason C. Porta ◽  
Jessica L. Hanks ◽  
Yelena Peskova ◽  
Elad Binshtein ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Membrane Protein ◽  
Single Particle ◽  
Structural Organization ◽  
Building Blocks ◽  
Structural Features ◽  
Current Evidence ◽  
New Model ◽  
Caveolin 1 ◽  
Single Particle Electron Microscopy

AbstractHighly stable oligomeric complexes of the monotopic membrane protein caveolin serve as fundamental building blocks of caveolae. Current evidence suggests these complexes are disc shaped, but the details of their structural organization and how they assemble are poorly understood. Here, we address these questions using single particle electron microscopy of negatively stained recombinant 8S complexes of human Caveolin-1. We show that 8S complexes are toroidal structures ~15 nm in diameter that consist of an outer ring, an inner ring, and central protruding stalk. Moreover, we map the position of the N- and C-termini and determine their role in complex assembly, and visualize the 8S complexes in heterologous caveolae. Our findings provide critical insights into the structural features of 8S complexes and allow us to propose a new model for how these highly stable membrane-embedded complexes are generated.

Modular Imaging Scaffold for Single-Particle Electron Microscopy

ACS Nano ◽  
2021 ◽  
Author(s):  
Nesrine Aissaoui ◽  
Josephine Lai-Kee-Him ◽  
Allan Mills ◽  
Nathalie Declerck ◽  
Zakia Morichaud ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy

scholarly journals Deblurring of class-averaged images in single-particle electron microscopy

2010 ◽  
Vol 26 (3) ◽  
pp. 035002 ◽  
Author(s):  
Wooram Park ◽  
Dean R Madden ◽  
Daniel N Rockmore ◽  
Gregory S Chirikjian
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy

scholarly journals Single particle electron microscopy

2009 ◽  
Vol 102 (2-3) ◽  
pp. 189-196 ◽  
Author(s):  
Egbert J. Boekema ◽  
Mihaela Folea ◽  
Roman Kouřil
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy

scholarly journals Seeing the insulin receptor in action

2018 ◽  
Vol 217 (5) ◽  
pp. 1555-1555
Author(s):  
Ben Short
Keyword(s):  
Electron Microscopy ◽  
Insulin Receptor ◽  
Single Particle ◽  
Receptor Activation ◽  
Single Particle Electron Microscopy

Researchers use single-particle electron microscopy to visualize an insulin-induced conformational shift that leads to receptor activation.

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