Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling

2019 ◽  
pp. 199-217 ◽  
Author(s):  
Gretchen L. Seim ◽  
Emily C. Britt ◽  
Jing Fan
Keyword(s):  
Isotopic Labeling ◽  
Arginine Metabolism

Arginine metabolism and gut microbiota changes in pediatric patients with type 1 diabetes

2020 ◽  
Author(s):  
Elena Aghajanova ◽  
Arthur Melkonyan ◽  
Nina Alchujyan ◽  
Bayburdyan Gayane ◽  
Margarita Hovhannisyan ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Gut Microbiota ◽  
Pediatric Patients ◽  
Arginine Metabolism

scholarly journals ARGININE METABOLISM AND ARTERIAL COMPLIANCE IN HYPERTENSION: THE EFFECT OF NEBIVOLOL THERAPY

2021 ◽  
Vol 77 (18) ◽  
pp. 1857
Author(s):  
Camilo Fernandez - Alonso ◽  
G. Elizabeth Smith ◽  
Alexander Razavi ◽  
Louise Roffidal
Keyword(s):  
Arterial Compliance ◽  
Arginine Metabolism

scholarly journals Argininosuccinate synthase 1 suppresses tumor progression through activation of PERK/eIF2α/ATF4/CHOP axis in hepatocellular carcinoma

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Sanghwa Kim ◽  
Minji Lee ◽  
Yeonhwa Song ◽  
Su-Yeon Lee ◽  
Inhee Choi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Er Stress ◽  
Stress Responses ◽  
Arginine Metabolism ◽  
Tumor Spheroids ◽  
Clinical Value ◽  
Suppressor Activity ◽  
Argininosuccinate Synthase ◽  
Hcc Cells

Abstract Background Hepatocellular carcinoma (HCC) is one of the most common malignant cancers worldwide, and liver cancer has increased in mortality due to liver cancer because it was detected at an advanced stages in patients with liver dysfunction, making HCC a lethal cancer. Accordingly, we aim to new targets for HCC drug discovery using HCC tumor spheroids. Methods Our comparative proteomic analysis of HCC cells grown in culture as monolayers (2D) and spheroids (3D) revealed that argininosuccinate synthase 1 (ASS1) expression was higher in 3D cells than in 2D cells due to upregulated endoplasmic reticulum (ER) stress responses. We investigated the clinical value of ASS1 in Korean patients with HCC. The mechanism underlying ASS1-mediated tumor suppression was investigated in HCC spheroids. ASS1-mediated improvement of chemotherapy efficiency was observed using high content screening in an HCC xenograft mouse model. Results Studies of tumor tissue from Korean HCC patients showed that, although ASS1 expression was low in most samples, high levels of ASS1 were associated with favorable overall survival of patients. Here, we found that bidirectional interactions between ASS1 ER stress responses in HCC-derived multicellular tumor spheroids can limit HCC progression. ASS1 overexpression effectively inhibited tumor growth and enhanced the efficacy of in vitro and in vivo anti-HCC combination chemotherapy via activation of the PERK/eIF2α/ATF4/CHOP axis, but was not dependent on the status of p53 and arginine metabolism. Conclusions These results demonstrate the critical functional roles for the arginine metabolism–independent tumor suppressor activity of ASS1 in HCC and suggest that upregulating ASS1 in these tumors is a potential strategy in HCC cells with low ASS1 expression.

scholarly journals Regulation of arginine metabolism in Saccharomyces cerevisiae. Association of arginase and ornithine transcarbamoylase.

1986 ◽  
Vol 261 (27) ◽  
pp. 12814-12819 ◽  
Author(s):  
E Eisenstein ◽  
L T Duong ◽  
R L Ornberg ◽  
J C Osborne ◽  
P Hensley
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Arginine Metabolism ◽  
Ornithine Transcarbamoylase

Phosphorus Dynamics in a Highly Weathered Soil as Revealed by Isotopic Labeling Techniques

2004 ◽  
Vol 68 (5) ◽  
pp. 1645-1655 ◽  
Author(s):  
E. K. Bünemann ◽  
F. Steinebrunner ◽  
P. C. Smithson ◽  
E. Frossard ◽  
A. Oberson
Keyword(s):  
Isotopic Labeling ◽  
Phosphorus Dynamics ◽  
Weathered Soil

Melatonin attenuates Leishmania (L.) amazonensis infection by modulating arginine metabolism

2015 ◽  
Vol 59 (4) ◽  
pp. 478-487 ◽  
Author(s):  
Maria Fernanda Laranjeira-Silva ◽  
Ricardo A. Zampieri ◽  
Sandra M. Muxel ◽  
Lucile Maria Floeter-Winter ◽  
Regina P. Markus
Keyword(s):  
Arginine Metabolism
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