DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea

Author(s):  
Xiuran Yin ◽  
Ajinkya C. Kulkarni ◽  
Michael W. Friedrich
2017 ◽  
Vol 202 ◽  
pp. 36-42 ◽  
Author(s):  
Jiangbing Xu ◽  
Zhongjun Jia ◽  
Xiangui Lin ◽  
Youzhi Feng

2011 ◽  
Vol 77 (11) ◽  
pp. 3749-3756 ◽  
Author(s):  
Sabrina Beckmann ◽  
Tillmann Lueders ◽  
Martin Krüger ◽  
Frederick von Netzer ◽  
Bert Engelen ◽  
...  

ABSTRACTIn abandoned coal mines, methanogenic archaea are responsible for the production of substantial amounts of methane. The present study aimed to directly unravel the active methanogens mediating methane release as well as active bacteria potentially involved in the trophic network. Therefore, the stable-isotope-labeled precursors of methane, [13C]acetate and H2-13CO2, were fed to liquid cultures from hard coal and mine timber from a coal mine in Germany. Guided by methane production rates, samples for DNA stable-isotope probing (SIP) with subsequent quantitative PCR and denaturing gradient gel electrophoretic (DGGE) analyses were taken over 6 months. Surprisingly, the formation of [13C]methane was linked to acetoclastic methanogenesis in both the [13C]acetate- and the H2-13CO2-amended cultures of coal and timber. H2-13CO2was used mainly by acetogens related toPelobacter acetylenicusandClostridiumspecies. Active methanogens, closely affiliated withMethanosarcina barkeri, utilized the readily available acetate rather than the thermodynamically more favorable hydrogen. Thus, the methanogenic microbial community appears to be highly adapted to the low-H2conditions found in coal mines.


2021 ◽  
pp. 117505
Author(s):  
Huanjun Zhang ◽  
Yin Lu ◽  
Yi Li ◽  
Lei Wang ◽  
Wenlong Zhang ◽  
...  

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