Faculty Opinions recommendation of Functional characterization of an anaerobic benzene-degrading enrichment culture by DNA stable isotope probing.

2010 ◽  
Author(s):  
Ian M Head ◽  
Casey Hubert
Keyword(s):  
Stable Isotope ◽  
Enrichment Culture ◽  
Functional Characterization ◽  
Stable Isotope Probing

scholarly journals Functional characterization of an anaerobic benzene-degrading enrichment culture by DNA stable isotope probing

2010 ◽  
Vol 12 (2) ◽  
pp. 401-411 ◽  
Author(s):  
Steffi Herrmann ◽  
Sabine Kleinsteuber ◽  
Antonis Chatzinotas ◽  
Steffen Kuppardt ◽  
Tillmann Lueders ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Enrichment Culture ◽  
Functional Characterization ◽  
Stable Isotope Probing

scholarly journals Characterization of growing bacterial populations in McMurdo Dry Valley soils through stable isotope probing with18O-water

2014 ◽  
Vol 89 (2) ◽  
pp. 415-425 ◽  
Author(s):  
Egbert Schwartz ◽  
David J. Van Horn ◽  
Heather N. Buelow ◽  
Jordan G. Okie ◽  
Michael N. Gooseff ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Probing ◽  
Bacterial Populations ◽  
Dry Valley

RNA-Stable Isotope Probing v2

2021 ◽  
Author(s):  
Roey Angel ◽  
Eva Petrova ◽  
Ana Lara
Keyword(s):  
Stable Isotope ◽  
Nucleic Acids ◽  
Density Gradient ◽  
Environmental Sample ◽  
Environmental Samples ◽  
Enrichment Culture ◽  
Stable Isotope Probing ◽  
Recent Book ◽  
The Subject ◽  
Comprehensive Discussion

The following protocol describes how to perform an RNA-Stable Isotope Probing experiment. The scope of this protocol only covers the parts involving separating labelled RNA from unlabelled RNA using ultracentrifugation in a caesium trifluoroacetate density gradient and downstream quantification to evaluate whether the labelling and separation of the RNA were successful. Total RNA should be extracted from an environmental sample or an enrichment culture that was incubated with an isotopically-labelled substrate. Labelling can be of the carbon, oxygen or nitrogen in the RNA (or any combination of the 3). For environmental samples, we recommend extracting RNA using our protocol Total Nucleic Acids Extraction from Soil and purifying it using the Purification of RNA from Crude NA Extract protocol. This protocol is based on the following papers: Whiteley et al. (2007); Dumont et al. (2011); Angel and Conrad (2013). For a comprehensive discussion on how to design a SIP experiment and how to analyse the resulting data, we recommend referring to the recent book on the subject: Stable Isotope Probing: Methods and Protocols, especially chapters: 1-3 and 9-18.

RNA-Stable Isotope Probing v2

2021 ◽  
Author(s):  
Roey Angel ◽  
Eva Petrova ◽  
Ana Lara
Keyword(s):  
Stable Isotope ◽  
Nucleic Acids ◽  
Density Gradient ◽  
Environmental Sample ◽  
Environmental Samples ◽  
Enrichment Culture ◽  
Stable Isotope Probing ◽  
Recent Book ◽  
The Subject ◽  
Comprehensive Discussion

The following protocol describes how to perform an RNA-Stable Isotope Probing experiment. The scope of this protocol only covers the parts involving separating labelled RNA from unlabelled RNA using ultracentrifugation in a caesium trifluoroacetate density gradient and downstream quantification to evaluate whether the labelling and separation of the RNA were successful. Total RNA should be extracted from an environmental sample or an enrichment culture that was incubated with an isotopically-labelled substrate. Labelling can be of the carbon, oxygen or nitrogen in the RNA (or any combination of the 3). For environmental samples, we recommend extracting RNA using our protocol Total Nucleic Acids Extraction from Soil and purifying it using the Purification of RNA from Crude NA Extract protocol. This protocol is based on the following papers: Whiteley et al. (2007); Dumont et al. (2011); Angel and Conrad (2013). For a comprehensive discussion on how to design a SIP experiment and how to analyse the resulting data, we recommend referring to the recent book on the subject: Stable Isotope Probing: Methods and Protocols, especially chapters: 1-3 and 9-18.

scholarly journals Functional analysis of an anaerobic m-xylene-degrading enrichment culture using protein-based stable isotope probing

2012 ◽  
Vol 81 (1) ◽  
pp. 134-144 ◽  
Author(s):  
Dragana Bozinovski ◽  
Steffi Herrmann ◽  
Hans-Hermann Richnow ◽  
Martin Bergen ◽  
Jana Seifert ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Stable Isotope ◽  
Enrichment Culture ◽  
Stable Isotope Probing
Sign in / Sign up

Export Citation Format

Share Document