Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging

2019 ◽  
pp. 103-111 ◽  
Author(s):  
Dhaka Ram Bhandari ◽  
Giulia Coliva ◽  
Maria Fedorova ◽  
Bernhard Spengler
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Atmospheric Pressure ◽  
Single Cell Analysis ◽  
Maldi Ms ◽  
Cell Analysis ◽  
Ms Imaging ◽  
Atmospheric Pressure Maldi

scholarly journals μCB-seq: Microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells

2020 ◽  
Author(s):  
Tyler N. Chen ◽  
Anushka Gupta ◽  
Mansi Zalavadia ◽  
Aaron M. Streets
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Protein Localization ◽  
Single Cells ◽  
Optical Measurements ◽  
Detection Sensitivity ◽  
High Resolution Imaging ◽  
Cell Analysis ◽  
Resolution Imaging

AbstractSingle-cell RNA sequencing (scRNA-seq) enables the investigation of complex biological processes in multicellular organisms with high resolution. However, many phenotypic features that are critical to understanding the functional role of cells in a heterogeneous tissue or organ are not directly encoded in the genome and therefore cannot be profiled with scRNA-seq. Quantitative optical microscopy has long been a powerful approach for characterizing diverse cellular phenotypes including cell morphology, protein localization, and chemical composition. Combining scRNA-seq with optical imaging has the potential to provide comprehensive single-cell analysis, allowing for functional integration of gene expression profiling and cell-state characterization. However, it is difficult to track single cells through both measurements; therefore, coupling current scRNA-seq protocols with optical measurements remains a challenge. Here, we report Microfluidic Cell Barcoding and Sequencing (μCB-seq), a microfluidic platform that combines high-resolution imaging and sequencing of single cells. μCB-seq is enabled by a novel fabrication method that preloads primers with known barcode sequences inside addressable reaction chambers of a microfluidic device. In addition to enabling multi-modal single-cell analysis, μCB-seq improves gene detection sensitivity, providing a scalable and accurate method for information-rich characterization of single cells.

scholarly journals Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells

The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3226-3238 ◽  
Author(s):  
Jitraporn Vongsvivut ◽  
David Pérez-Guaita ◽  
Bayden R. Wood ◽  
Philip Heraud ◽  
Karina Khambatta ◽  
...  
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Spatial Resolution ◽  
Environmental Science ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Cell Analysis ◽  
Chemical Mapping ◽  
Ftir Microspectroscopy ◽  
Resolution Single

Coupling synchrotron IR beam to an ATR element enhances spatial resolution suited for high-resolution single cell analysis in biology, medicine and environmental science.

scholarly journals Chromatinin situproximity (ChrISP): Single-cell analysis of chromatin proximities at a high resolution

BioTechniques ◽  
2014 ◽  
Vol 56 (3) ◽  
Author(s):  
Xingqi Chen ◽  
Chengxi Shi ◽  
Samer Yammine ◽  
Anita Göndör ◽  
Daniel Rönnlund ◽  
...  
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis
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