scholarly journals Assembly of Immature HIV-1 Capsids Using a Cell-Free System

2009 ◽  
pp. 185-195 ◽  
Author(s):  
Jaisri R. Lingappa ◽  
Beth K. Thielen
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Hiv 1

Interaction of HIV-1 Gag and Membranes in a Cell-Free System

Virology ◽  
2002 ◽  
Vol 302 (1) ◽  
pp. 164-173 ◽  
Author(s):  
Liuzhan Yang ◽  
Lee Ratner
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Hiv 1

In Vitro HIV-1 Infection of Isolated Epidermal Langerhans Cells with a Cell-Free System

1995 ◽  
pp. 465-468 ◽  
Author(s):  
Anne-Sophie Charbonnier ◽  
François Mallet ◽  
Marie-Madeleine Fiers ◽  
Claude Desgranges ◽  
Colette Dezutter-Dambuyant ◽  
...  
Keyword(s):  
Langerhans Cells ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Hiv 1 ◽  
Epidermal Langerhans Cells

scholarly journals Conservation of a Stepwise, Energy-Sensitive Pathway Involving HP68 for Assembly of Primate Lentivirus Capsids in Cells

2004 ◽  
Vol 78 (4) ◽  
pp. 1645-1656 ◽  
Author(s):  
Julia E. Dooher ◽  
Jaisri R. Lingappa
Keyword(s):  
Capsid Assembly ◽  
Free System ◽  
Cell Free System ◽  
Gag Proteins ◽  
Sensitivity Pattern ◽  
Immunodeficiency Virus ◽  
A Cell ◽  
Energy Dependent ◽  
Hiv 1 ◽  
In Cells

ABSTRACT Previously we have described a stepwise, energy-dependent pathway for human immunodeficiency virus type 1 (HIV-1) capsid assembly in a cell-free system. In this pathway, Gag polypeptides utilize the cellular factor HP68 and assemble into immature capsids by way of assembly intermediates that have defined biochemical characteristics. Here we address whether this pathway is universally conserved among primate lentiviruses and can be observed in mammalian cells. We demonstrate that HIV-2 Gag associates with human HP68 in a cell-free system and that Gag proteins of HIV-2, simian immunodeficiency virus SIVmac239, and SIVagm associate with endogenous HP68 in primate cells, as is seen for HIV-1. Analysis of primate cells expressing lentivirus Gag proteins revealed Gag-containing complexes with the same sedimentation values as seen for previously described HIV-1 assembly intermediates in the cell-free system (10S, 80-150S, and 500S). These complexes fit criteria for assembly intermediates as judged by energy sensitivity, pattern of HP68 association, and the failure of specific complexes to be formed by assembly-incompetent Gag mutants. We also demonstrate that virus-like particles released from cells do not appear to contain HP68, suggesting that HP68 is released from Gag upon completion of capsid assembly in cells, as was observed previously in the cell-free system. Together these findings support a model in which all primate lentivirus capsids assemble by a conserved pathway of HP68-containing, energy-dependent assembly intermediates that have specific biochemical features.

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell
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