Mouse Models for miRNA Expression: The ROSA26 Locus

2010 ◽  
pp. 145-163 ◽  
Author(s):  
Stefano Casola
Keyword(s):  
Mouse Models ◽  
Mirna Expression ◽  
Rosa26 Locus

Hepatic miRNA expression profiling in genetically modified mouse models of different atherosclerosis susceptibility

2016 ◽  
Vol 252 ◽  
pp. e206-e207
Author(s):  
S. Manzini ◽  
M. Busnelli ◽  
M. Chiara ◽  
C. Parolini ◽  
F. Dellera ◽  
...  
Keyword(s):  
Mouse Models ◽  
Mirna Expression ◽  
Expression Profiling ◽  
Genetically Modified ◽  
Mirna Expression Profiling

Characterization of New Transgenic Mouse Models for Two Charcot-Marie-Tooth-Causing HspB1 Mutations using the Rosa26 Locus

2016 ◽  
Vol 3 (2) ◽  
pp. 183-200 ◽  
Author(s):  
Delphine Bouhy ◽  
Thomas Geuens ◽  
Vicky De Winter ◽  
Leonardo Almeida-Souza ◽  
Istvan Katona ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Models ◽  
Transgenic Mouse Models ◽  
Rosa26 Locus ◽  
Charcot Marie Tooth

scholarly journals Comprehensive analysis of microRNA signature of mouse pancreatic acini: overexpression of miR-21-3p in acute pancreatitis

2016 ◽  
Vol 311 (5) ◽  
pp. G974-G980 ◽  
Author(s):  
Ajay Kumar Dixit ◽  
Anne E. Sarver ◽  
Zuobiao Yuan ◽  
John George ◽  
Usman Barlass ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Mouse Models ◽  
Expression Profile ◽  
Mirna Expression ◽  
Acinar Cells ◽  
Mirna Expression Profile ◽  
Comprehensive Analysis ◽  
Pancreatic Acinar Cells ◽  
Pancreatic Acini ◽  
Severity Of The Disease

In the current study, we have characterized the global miRNA expression profile in mouse pancreatic acinar cells and during acute pancreatitis using next-generation RNA sequencing. We identified 324 known and six novel miRNAs that are expressed in mouse pancreatic acinar cells. In the basal state, miR-148a-3p, miR-375-3p, miR-217-5p, and miR-200a-3p were among the most abundantly expressed, whereas miR-24-5p and miR-421-3p were the least abundant. Treatment of acinar cells with caerulein (100 nM) and taurolithocholic acid 3-sulfate [TLC-S (250 μM)] induced numerous changes in miRNA expression profile. In particular, we found significant overexpression of miR-21-3p in acini treated with caerulein and TLC-S. We further looked at the expression of miR-21-3p in caerulein, l-arginine, and caerulein + LPS-induced acute pancreatitis mouse models and found 12-, 21-, and 50-fold increased expression in the pancreas, respectively. In summary, this is the first comprehensive analysis of global miRNA expression profile of mouse pancreatic acinar cells in normal and disease conditions. Our analysis shows that miR-21-3p expression level correlates with the severity of the disease.

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