DIETARY INTAKE AND SUN EXPOSURE RELATED TO VITAMIN D CONCENTRATION IN THALASSEMIA PATIENTS: A LITERATURE REVIEW

Vitamin D has an important role in calcium homeostasis and bone minerals during rapid growth periods. Several studies have shown that deficiency of vitamin D occurs in thalassemia patient. The study used literature review to determine relation of dietary intake and sun exposure with vitamin D concentration in thalassemia patiens in 29 literatures. Those literatures were taken from books and articles published from 2010 to 2019 with the keywords “thalassemia”, “dietary intake”, “sun exposure” and “vitamin D” using database in Pubmed, Google Scholar and Medline. The results of 29 literatures showed that vitamin D deficiency is caused by reduced dietary intake and impaired vitamin D hydroxylation in the liver due to hemochromatosis resulting in high serum ferritin. Source of vitamin D comes from endogenous synthesis with sunlight exposure and little dietary source of vitamin D2 and vitamin D3. Another food intake can also affect serum vitamin D concentration, mainly fat and protein intake. Vitamin D is fat soluble vitamin, it can be stored in the fat for later metabolized in the liver. Protein is required to transport vitamin D to blood circulation, enzyme formation and vitamin D receptor (VDR). Thalassemia patients need to increase of macro and micronutrients requirement. Low Hb concentration causes fatigue, tired easily and decreased appetite. A lot of research on thalassemia children found that intake of energy and protein were lower than recommended and lack of sun exposure. These conditions will affect to vitamin D concentration. A comprehensive understanding in the relationship of dietary intake and sun exposure to vitamin D concentration in thalassemia patients is explained in this mini review. Maintaining normal vitamin D concentration through adequate dietary intake and sun exposure are very important to optimize growth in thalassemia patients.

Nano-Hydroxyapatite as A Delivery System for Promoting Bone Regeneration In Vivo: A Systematic Review

Nano-hydroxyapatite (nHA) has been widely used as an orthopedic biomaterial and vehicle for drug delivery owing to its chemical and structural similarity to bone minerals. Several studies have demonstrated that nHA based biomaterials have a potential effect for bone regeneration with very minimal to no toxicity or inflammatory response. This systematic review aims to provide an appraisal of the effectiveness of nHA as a delivery system for bone regeneration and whether the conjugation of proteins, antibiotics, or other bioactive molecules to the nHA further enhances osteogenesis in vivo. Out of 282 articles obtained from the literature search, only 14 articles met the inclusion criteria for this review. These studies showed that nHA was able to induce bone regeneration in various animal models with large or critical-sized bone defects, open fracture, or methicillin-resistant Staphylococcus aureus (MRSA)-induced osteomyelitis. The conjugations of drugs or bioactive molecules such as bone-morphogenetic protein-2 (BMP-2), vancomycin, calcitriol, dexamethasone, and cisplatin were able to enhance the osteogenic property of nHA. Thus, nHA is a promising delivery system for a variety of compounds in promoting bone regeneration in vivo.

Characteristics of Biodegradable Gelatin Methacrylate Hydrogel Designed to Improve Osteoinduction and Effect of Additional Binding of Tannic Acid on Hydrogel

In this study, a hydrogel using single and double crosslinking was prepared using GelMA, a natural polymer, and the effect was evaluated when the double crosslinked hydrogel and tannic acid were treated. The resulting hydrogel was subjected to physicochemical property evaluation, biocompatibility evaluation, and animal testing. The free radicals generated through APS/TEMED have a scaffold form with a porous structure in the hydrogel, and have a more stable structure through photo crosslinking. The double crosslinked hydrogel had improved mechanical strength and better results in cell compatibility tests than the single crosslinked group. Moreover, in the hydrogel transplanted into the femur of a rat, the double crosslinked group showed an osteoinductive response due to the attachment of bone minerals after 4 and 8 weeks, but the single crosslinked group did not show an osteoinductive response due to rapid degradation. Treatment with a high concentration of tannic acid showed significantly improved mechanical strength through H-bonding. However, cell adhesion and proliferation were limited compared to the untreated group due to the limitation of water absorption capacity, and no osteoinduction reaction was observed. As a result, it was confirmed that the treatment of high-concentration tannic acid significantly improved mechanical strength, but it was not a suitable method for improving bone induction due to the limitation of water absorption.

Body composition of Tashkent school-aged boys

Background: Body composition in periods of childhood, puberty and adolescent is a marker of metabolic changes that occur during these periods of growth and maturation, and, thus, it is an important information regarding child and adolescent health. Purpose: The purpose is to study the age-related body index mass and body composition in the different age school boys in Tashkent city. Method: Boys body composition was measured by analyzer “Tanita BC 543” (Japan) in the morning between 9.00-10.00 p.m. Body composition parameters were determined in boys of three stage of development: childhood (seven year old), early adolescence (twelve year old), and late adolescence age (eighteen year old). Results: The obtained data show that as boys grow and develop, mass of all body components increased. The greatest increase was in adipose tissue, and the least one was in content of bone minerals. It was found 7-year-old children were underweight, but by the age of 18 there weight approached the normal value. However, weight normalization occurs due to an increase in adipose tissue. It is indirectly indicated a growing risk in the boys predisposition to the diabetes.

Palmul-Tang, a Korean Medicine, Promotes Bone Formation via BMP-2 Pathway in Osteoporosis

Osteoporosis is a common skeletal disease in post-menopausal women. Palmul-tang, an herbal medicine, has been treated for gynecological disease such as anemia, anorexia, anti-fatigue, unspecified menstruation and female infertility in East Asia. In this study, ameliorative effects of Palmul-tang soft extracts (PMT), a Korean Medicine, on osteoporosis were investigated. Ovariectomized (OVX) osteoporotic ICR mice were intragastrically administrated PMT for 4 weeks. The level of bone mineral density (BMD) was analyzed in bone tissues by dual X-ray absorptiometry. The bone medullary cavity and deposition of collagen were investigated by histological analysis. In addition, the BMP-2 signaling-related molecules, osteoblastic differentiation and formation markers, were determined in femoral tissues. The levels of BMD and bone mineral content were significantly increased in tibia, femurs and LV by treatment of PMT. PMT replenished bone marrow cavity and increased collagen deposition in bone marrow cells of femur. In addition, administration of PMT recovered serum ALP, bALP, osteocalcin and calcium levels in osteoporotic mice. Moreover, PMT treatment up-regulated the expressions of BMP-2, RUNX2 and OSX with its downstream factors, ALP, OPN and BSP-1, in the femoral tissues. Taken together, PMT restored the bone minerals and improvement of bone integrity by bone-forming BMP-2 signaling pathway. These results demonstrate that PMT could be an ameliorative agent for osteoporosis.

Laporan Kasus: Ekstraksi Gigi dengan Perubahan Matriks Tulang sebagai Persiapan Pembuatan Gigi Tiruan Lengkap

Patients in the elderly group have a higher risk for dental and oral health problems because in old age, caries and periodontal tissue are generally more severe and progressive. The aging process causes changes in the structure and appearance of the teeth, the oral mucosa, the decline in function salivary glands, and loss of bone minerals through bone matrix resorption. The occurrence of bone matrix resorption results in the elderly group more often experiencing tooth loss which can be exacerbated by the occurrence of periodontal disease. After tooth extraction, this condition needs to be considered by dentist so that the denture made later has good retention and stabilization and remains comfortable for the patient to use. The aim was providing an overview regarding the procedure of extraction teeth with the change of bone matrix as a preparation for the prosthodontic complete dentures treatment. A 56-year-old female patient came to RSGM Unsoed wanted to order complete dentures so that the remaining teeth shoul be extracted. Patient feels discomfort and difficulties when eating and drinking. Intraoral examination, only teeth 16 were left with gingival recession. The patient's alveolar bone condition showst ends to be flat in the edentulous posterior area. The extraction case in elderly patients must be carried out according to the right procedure with minimal trauma so as not to damage the supporting tissue around the tooth and facilitate the process of denture treatment.