Strain Engineering Strategies for Improving Whole-Cell Biocatalysis: Engineering Escherichia coli to Overproduce Xylitol as an Example

2011 ◽  
pp. 185-203 ◽  
Author(s):  
Jonathan W. Chin ◽  
Patrick C. Cirino
Keyword(s):  
Escherichia Coli ◽  
Strain Engineering ◽  
Whole Cell ◽  
Whole Cell Biocatalysis

scholarly journals Using Chemobiosynthesis and Synthetic Mini-Polyketide Synthases To Produce Pharmaceutical Intermediates in Escherichia coli

2010 ◽  
Vol 76 (15) ◽  
pp. 5221-5227 ◽  
Author(s):  
Hugo G. Menzella ◽  
John R. Carney ◽  
Yong Li ◽  
Daniel V. Santi
Keyword(s):  
Escherichia Coli ◽  
Therapeutic Agents ◽  
Polyketide Synthases ◽  
Manufacturing Cost ◽  
Commercial Development ◽  
Complex Molecules ◽  
Whole Cell ◽  
Enantiomerically Pure ◽  
Whole Cell Biocatalysis ◽  
Stereogenic Centers

ABSTRACT Recombinant microbial whole-cell biocatalysis is a valuable approach for producing enantiomerically pure intermediates for the synthesis of complex molecules. Here, we describe a method to produce polyketide intermediates possessing multiple stereogenic centers by combining chemobiosynthesis and engineered mini-polyketide synthases (PKSs). Chemobiosynthesis allows the introduction of unnatural moieties, while a library of synthetic bimodular PKSs expressed from codon-optimized genes permits the introduction of a variety of ketide units. To validate the approach, intermediates for the synthesis of trans-9,10-dehydroepothilone D were generated. The designer molecules obtained have the potential to greatly reduce the manufacturing cost of epothilone analogues, thus facilitating their commercial development as therapeutic agents.

scholarly journals Engineering a pyridoxal 5’-phosphate supply for cadaverine production by using Escherichia coli whole-cell biocatalysis

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Weichao Ma ◽  
Weijia Cao ◽  
Bowen Zhang ◽  
Kequan Chen ◽  
Quanzhen Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Cell ◽  
Whole Cell Biocatalysis ◽  
Phosphate Supply

scholarly journals Engineering NAD+ availability for Escherichia coli whole-cell biocatalysis: a case study for dihydroxyacetone production

2013 ◽  
Vol 12 (1) ◽  
pp. 103 ◽  
Author(s):  
Yongjin J Zhou ◽  
Wei Yang ◽  
Lei Wang ◽  
Zhiwei Zhu ◽  
Sufang Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Cell ◽  
Whole Cell Biocatalysis

scholarly journals Enzymatic Synthesis of Novel Phloretin Glucosides

2013 ◽  
Vol 79 (11) ◽  
pp. 3516-3521 ◽  
Author(s):  
Ramesh Prasad Pandey ◽  
Tai Feng Li ◽  
Eun-Hee Kim ◽  
Tokutaro Yamaguchi ◽  
Yong Il Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Licheniformis ◽  
Enzymatic Synthesis ◽  
Whole Cell ◽  
Content Type ◽  
Whole Cell Biocatalysis ◽  
Glycosylation Reaction

ABSTRACTA UDP-glycosyltransferase fromBacillus licheniformiswas exploited for the glycosylation of phloretin. Thein vitroglycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineeredEscherichia coli.

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