enzymatic synthesis
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Catalysts ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 88
Author(s):  
Adejanildo da S. Pereira ◽  
Aline Habibe de Souza ◽  
Jully L. Fraga ◽  
Pierre Villeneuve ◽  
Alexandre G. Torres ◽  
...  

Lipases are versatile enzymes widely used in the pharmaceutical, cosmetic, and food industries. They are green biocatalysts with a high potential for industrial use compared to traditional chemical methods. In recent years, lipases have been used to synthesize a wide variety of molecules of industrial interest, and extraordinary results have been reported. In this sense, this review describes the important role of lipases in the synthesis of phytosterol esters, which have attracted the scientific community’s attention due to their beneficial effects on health. A systematic search for articles and patents published in the last 20 years with the terms “phytosterol AND esters AND lipase” was carried out using the Scopus, Web of Science, Scielo, and Google Scholar databases, and the results showed that Candida rugosa lipases are the most relevant biocatalysts for the production of phytosterol esters, being used in more than 50% of the studies. The optimal temperature and time for the enzymatic synthesis of phytosterol esters mainly ranged from 30 to 101 °C and from 1 to 72 h. The esterification yield was greater than 90% for most analyzed studies. Therefore, this manuscript presents the new technological approaches and the gaps that need to be filled by future studies so that the enzymatic synthesis of phytosterol esters is widely developed.


2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Wataru Saburi ◽  
Takanori Nihira ◽  
Hiroyuki Nakai ◽  
Motomitsu Kitaoka ◽  
Haruhide Mori

AbstractGlycoside phosphorylases (GPs), which catalyze the reversible phosphorolysis of glycosides, are promising enzymes for the efficient production of glycosides. Various GPs with new catalytic activities are discovered from uncharacterized proteins phylogenetically distant from known enzymes in the past decade. In this study, we characterized Paenibacillus borealis PBOR_28850 protein, belonging to glycoside hydrolase family 94. Screening of acceptor substrates for reverse phosphorolysis, in which α-d-glucose 1-phosphate was used as the donor substrate, revealed that the recombinant PBOR_28850 produced in Escherichia coli specifically utilized d-galactose as an acceptor and produced solabiose (β-d-Glcp-(1 → 3)-d-Gal). This indicates that PBOR_28850 is a new GP, solabiose phosphorylase. PBOR_28850 catalyzed the phosphorolysis and synthesis of solabiose through a sequential bi-bi mechanism involving the formation of a ternary complex. The production of solabiose from lactose and sucrose has been established. Lactose was hydrolyzed to d-galactose and d-glucose by β-galactosidase. Phosphorolysis of sucrose and synthesis of solabiose were then coupled by adding sucrose, sucrose phosphorylase, and PBOR_28850 to the reaction mixture. Using 210 mmol lactose and 280 mmol sucrose, 207 mmol of solabiose was produced. Yeast treatment degraded the remaining monosaccharides and sucrose without reducing solabiose. Solabiose with a purity of 93.7% was obtained without any chromatographic procedures.


Author(s):  
Julia Cristina ZUIN ◽  
Renata Luana de Pádua GANDRA ◽  
Ana Paula Badan RIBEIRO ◽  
Juliana Neves Rodrigues RACT ◽  
Juliana Alves MACEDO ◽  
...  

Author(s):  
Yukiko Takakura ◽  
Shintaro Arai ◽  
Kenji Kanaori ◽  
Hideyuki Suzuki
Keyword(s):  

Pharmaceutics ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 115
Author(s):  
Maia Merlani ◽  
Dieter M. Scheibel ◽  
Vakhtang Barbakadze ◽  
Lali Gogilashvili ◽  
Lela Amiranashvili ◽  
...  

This study reports the first enzymatic synthesis leading to several oligomer analogues of poly[3-(3,4-dihydroxyphenyl)glyceric acid]. This biopolymer, extracted from plants of the Boraginaceae family has shown a wide spectrum of pharmacological properties, including antimicrobial activity. Enzymatic ring opening polymerization of 2-methoxycarbonyl-3-(3,4-dibenzyloxyphenyl)oxirane (MDBPO) using lipase from Candida rugosa leads to formation of poly[2-methoxycarbonyl-3-(3,4-dibenzyloxyphenyl)oxirane] (PMDBPO), with a degree of polymerization up to 5. Catalytic debenzylation of PMDBPO using H2 on Pd/C yields poly[2-methoxycarbonyl-3-(3,4-dihydroxyphenyl)oxirane] (PMDHPO) without loss in molecular mass. Antibacterial assessment of natural polyethers from different species of Boraginaceae family Symhytum asperum, S. caucasicum,S. grandiflorum, Anchusa italica, Cynoglossum officinale, and synthetic polymers, poly[2-methoxycarbonyl-3-(3,4-dimethoxyphenyl)oxirane (PMDMPO) and PMDHPO, reveals that only the synthetic analogue produced in this study (PMDHPO) exhibits a promising antimicrobial activity against pathogenic strains S.aureus ATCC 25923 and E.coli ATCC 25922 the minimum inhibitory concentration (MIC) being 100 µg/mL.


2022 ◽  
pp. 111-122
Author(s):  
Karime de M. Moctezuma-Dávila ◽  
Ricardo D. Aguilar-García ◽  
Iris R. Cuellar-Rincón ◽  
Jorge E. Wong-Paz ◽  
Pedro Aguilar-Zárate ◽  
...  

2022 ◽  
pp. 253-267
Author(s):  
Daniela A. Gonçalves ◽  
José A. Teixeira ◽  
Clarisse Nobre
Keyword(s):  

RSC Advances ◽  
2022 ◽  
Vol 12 (4) ◽  
pp. 2310-2318
Author(s):  
Huan Li ◽  
Feng Qin ◽  
Lijuan Huang ◽  
Wenjing Jia ◽  
Mingliang Zhang ◽  
...  

Although water is an ideal green solvent for organic synthesis, it is difficult for most biocatalysts to carry out transesterification reactions in water because of the reversible hydrolysis reaction.


2022 ◽  
pp. 699-756
Author(s):  
Anindita Behera ◽  
Sweta Priyadarshini Pradhan ◽  
Farah K. Ahmed ◽  
Kamel A. Abd-Elsalam

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