Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins

2012 ◽  
pp. 111-118 ◽  
Author(s):  
Marion L. Greaser ◽  
Chad M. Warren
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Protein Electrophoresis ◽  
Agarose Gels ◽  
High Molecular Weight Proteins

scholarly journals Ein makroporöses Polyacrylamid für immunologische Diffusionstechniken / Macroporous Polyacrylamide, its Use for Immunological Diffusion Techniques

1972 ◽  
Vol 27 (6) ◽  
pp. 683-688 ◽  
Author(s):  
Helmut Determann ◽  
Axel Walch
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Monomer Concentration ◽  
Agarose Gels ◽  
Γ Globulins ◽  
Sample Application ◽  
Total Monomer Concentration ◽  
High Molecular Weight Proteins ◽  
High Concentrations

Gel layers of crosslinked polyacrylamide with different porosities were synthesized and their properties compared. At high concentrations of bifunctional monomers macroporous gels were formed, one of which (total monomer concentration 14%, methylene-bis-acrylamide 40%) permitted even high molecular weight proteins (e. g. γ-globulins) to diffuse more readily than in 2 per cent agar or agarose gels. Different immunological techniques were modified for application with this polymer. It offers advantages especially with respect to porosity, storability and sample application for quantitative diffusion techniques.

INTERFERENCE BY SERUM PROTEINS WITH LH RADIOIMMUNOASSAY USING IMMUNOSORBENT

1973 ◽  
Vol 72 (2) ◽  
pp. 235-242 ◽  
Author(s):  
A. M. Reuter ◽  
J. C. Hendrick ◽  
J. Sulon ◽  
P. Franchimont
Keyword(s):  
Molecular Weight ◽  
Human Serum ◽  
High Molecular Weight ◽  
Serum Proteins ◽  
Void Volume ◽  
High Molecular Weight Proteins ◽  
Serum Fractionation ◽  
Covalently Bound

ABSTRACT The percentage of LH* bound to antibodies that have been covalently bound to cellulose is diminished in the presence of LH-free human serum and sera from various species of animals. Serum fractionation studies on Sephadex G 200 show that the greatest interference comes from the proteins eluted in the void volume i. e. the high molecular weight proteins. Specifically, the gamma M globulins and the α2-macroglobulins appear to play an important role, as demonstrated by tests in which these proteins were neutralized by gamma M and α2-macroglobulin antisera.

scholarly journals Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

2010 ◽  
Vol 49 (11) ◽  
pp. 1958-1962 ◽  
Author(s):  
Pierre Gans ◽  
Olivier Hamelin ◽  
Remy Sounier ◽  
Isabel Ayala ◽  
M. Asunción Durá ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Isotopic Labeling ◽  
Spectroscopic Studies ◽  
Methyl Groups ◽  
High Molecular Weight Proteins

High Molecular Weight Proteins of Tumor Nuclear Matrix

1990 ◽  
pp. 355-359 ◽  
Author(s):  
I. B. Zbarsky ◽  
S. N. Kuzmina ◽  
T. V. Buldyaeva ◽  
T. M. Bazarnova
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Nuclear Matrix ◽  
High Molecular Weight Proteins

Influence of PEGylation on the Release of Low and High Molecular-Weight Proteins from PVA Matrices

1999 ◽  
Vol 14 (4) ◽  
pp. 315-330 ◽  
Author(s):  
F. M. Veronese ◽  
C. Mammucari ◽  
P. Caliceti ◽  
O. Schiavon ◽  
S. Lora
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Molecular Weight Proteins

Competition between Low- and High-Molecular-Weight Proteins for Renal Tubular Uptake

Nephron ◽  
1987 ◽  
Vol 45 (2) ◽  
pp. 115-118 ◽  
Author(s):  
A. Bernard ◽  
C. Viau ◽  
A. Ouled ◽  
R. Lauwerys
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Molecular Weight Proteins ◽  
Renal Tubular
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