Sperm Chromatin Structure Assay (SCSA®)

2012 ◽  
pp. 147-164 ◽  
Author(s):  
Donald P. Evenson
Keyword(s):  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay

Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Zygote ◽  
2003 ◽  
Vol 11 (4) ◽  
pp. 367-371 ◽  
Author(s):  
Isabelle Oger ◽  
Christelle Da Cruz ◽  
Gilles Panteix ◽  
Yves Menezo
Keyword(s):  
Dna Fragmentation ◽  
Chromatin Structure ◽  
Inverse Relationship ◽  
Sperm Concentration ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Oxidative Products ◽  
Dna Fragmentation Index

In our work, we have used 8-hydroxy-deoxyguanosine (8-OH-dG), one of the major oxidative products of sperm DNA, in a population of patients consulting for infertility. We found an inverse relationship between sperm concentration and the log of the ratio of 8-OH-dG to dG (P<0.01). On the same patients' sperm samples, the sperm chromatin structure assay (SCSA) was performed. An inverse relationship was observed between the DNA fragmentation index and sperm concentration (P<0.001). There was also a positive relationship between SCSA and log 8-OH-dG/dG. This indicates that DNA fragmentation measured by the SCSA originates in part from oxidative products. In a few patients, antioxidant treatment decreased the DNA fragmentation index below the threshold of 30% that is crucial for subfertility.

scholarly journals Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications

2006 ◽  
Vol 21 (8) ◽  
pp. 2061-2064 ◽  
Author(s):  
J. Erenpreiss ◽  
M. Bungum ◽  
M. Spano ◽  
S. Elzanaty ◽  
J. Orbidans ◽  
...  
Keyword(s):  
Chromatin Structure ◽  
Individual Variation ◽  
Sperm Chromatin ◽  
Clinical Implications ◽  
Sperm Chromatin Structure Assay ◽  
Infertile Couples

Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay

2002 ◽  
Vol 78 (2) ◽  
pp. 319-329 ◽  
Author(s):  
Juan G Alvarez ◽  
Rakesh K Sharma ◽  
Mario Ollero ◽  
Ramadan A Saleh ◽  
Mari C Lopez ◽  
...  
Keyword(s):  
Dna Damage ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay

The sperm chromatin structure assay (SCSA) and its relationship to IVF outcome: A prospective trial

2004 ◽  
Vol 82 ◽  
pp. S192 ◽  
Author(s):  
D.K. Gardner ◽  
W.B. Schoolcraft ◽  
E.S. Surrey ◽  
K. Stilley ◽  
J. Stevens ◽  
...  
Keyword(s):  
Chromatin Structure ◽  
Prospective Trial ◽  
Sperm Chromatin ◽  
Ivf Outcome ◽  
Sperm Chromatin Structure Assay

Prediction of Posttreatment Spermatogenesis in Patients With Testicular Cancer by Flow Cytometric Sperm Chromatin Structure Assay

1998 ◽  
pp. 947-948
Author(s):  
S. D. Fossa ◽  
P. De Angelis ◽  
S. M. Kraggerud ◽  
D. Evenson ◽  
L. Theodorsen ◽  
...  
Keyword(s):  
Testicular Cancer ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Flow Cytometric
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