Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones

2012 ◽  
pp. 123-148 ◽  
Author(s):  
Jon Brown ◽  
Atticus H. Hainsworth ◽  
Alessandro Stefani ◽  
Andrew D. Randall
Keyword(s):  
Patch Clamp ◽  
Heterologous Expression ◽  
Single Cells ◽  
Expression Systems ◽  
Patch Clamp Recording ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Heterologous Expression Systems ◽  
Channel Currents

Whole Cell Patch Clamp of Dispersed Human Islet Cells v1

2021 ◽  
Author(s):  
Xiaoqing Dai ◽  
Austin Bautista ◽  
Patrick E Macdonald
Keyword(s):  
Patch Clamp ◽  
Single Cell ◽  
Pancreatic Islets ◽  
Islet Cells ◽  
Single Cells ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Patch Clamp Electrophysiology ◽  
Channel Currents

Cells use exocytosis to secrete a wide variety of molecules, including proteins, hormones, and neurotransmitters. Exocytosis can be monitored at the single-cell level by using patch-clamp electrophysiology to measure changes in membrane capacitance as vesicles fuse with the cell membrane and release their content. Dispersion of pancreatic islets into single cells allows for individual characterization of electrophysiological characteristics and allows for collection of cellular content for recovery of full-length transcriptomes by use of Smart-seq2. Described in this protocol is the dispersion of pancreatic islets into single cells followed by whole-cell patch clamp electrophysiology which includes parameters representing cell size, exocytosis, sodium channel currents, and calcium channel currents. Cells are then collected individually after recording to be processed for single-cell RNA sequencing.

Error analysis of Cm measurement under the whole-cell patch-clamp recording

2010 ◽  
Vol 185 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Hao Zhang ◽  
Anlian Qu ◽  
Jie Luo ◽  
Jie Luo
Keyword(s):  
Patch Clamp ◽  
Error Analysis ◽  
Patch Clamp Recording ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp

Z f-and-H sys-based C m measurement under the whole-cell patch-clamp recording

2008 ◽  
Vol 457 (6) ◽  
pp. 1423-1434 ◽  
Author(s):  
Hao Zhang ◽  
Jie Luo ◽  
Jun Xiong ◽  
Xian-Guang Lin ◽  
Zheng-Xing Wu ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Patch Clamp Recording ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp

scholarly journals Whole Cell Patch Clamp Recording Performed on a Planar Glass Chip

2002 ◽  
Vol 82 (6) ◽  
pp. 3056-3062 ◽  
Author(s):  
Niels Fertig ◽  
Robert H. Blick ◽  
Jan C. Behrends
Keyword(s):  
Patch Clamp ◽  
Patch Clamp Recording ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Glass Chip
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