A probabilistic analysis method is developed for frequencies analysis of turbine blade with uncertain boundary condition at the root of blade. The Ritz method is used to derive the eigenvalues equation of the rotating blade with uncertain root boundary condition. The matrix perturbation technique is employed for the probabilistic analysis to obtain the deterministic part of natural frequencies and vibration modes, the sensitivity matrix, the covariance matrix and the coefficient of variance (COV) for the natural frequencies. The effects of variations in the expectation and the variance of joint stiffness on the expectation and the variance of the natural frequencies are investigated.