Fundamental Building Blocks of Whole-Cell Biosensor Design

2021 ◽  
pp. 383-405
Author(s):  
Ke Yan Wen ◽  
Jack W. Rutter ◽  
Chris P. Barnes ◽  
Linda Dekker
Author(s):  
Ke Yan Wen ◽  
Jack W. Rutter ◽  
Chris P. Barnes ◽  
Linda Dekker

2016 ◽  
Vol 52 (76) ◽  
pp. 11402-11405 ◽  
Author(s):  
Leopoldo F. M. Machado ◽  
Neil Dixon

To facilitate the screening of chemical, enzymatic, and cellular processes to degrade and valorize plant biomass a whole cell biosensor was developed to detect lignin-derived substrates.


Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 166
Author(s):  
Sergey Bazhenov ◽  
Uliana Novoyatlova ◽  
Ekaterina Scheglova ◽  
Vadim Fomin ◽  
Svetlana Khrulnova ◽  
...  

Aliivibrio fischeri LuxR and Aliivibrio logei LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of luxICDABEG genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the Aliivibrio cells, luxR genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the luxR expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection. Escherichia coli-based bacterial lux-biosensors were used, in which Photorhabdus luminescensluxCDABE genes were controlled by LuxR-dependent promoters and luxR, luxR1, or luxR2 regulatory genes. We varied either the dosage of the regulatory gene in the cells using additional plasmids, or the level of the regulatory gene expression using the lactose operon promoter. It was shown that an increase in expression level, as well as dosage of the regulatory gene in biosensor cells, leads to an increase in sensitivity (the threshold concentration of AI is reduced by one order of magnitude) and to a two to threefold reduction in response time. The best parameters were obtained for a biosensor with an increased dosage of luxRA. fischeri (sensitivity to 3-oxo-hexanoyl-l-homoserine lactone reached 30–100 pM).


2021 ◽  
Vol 12 ◽  
Author(s):  
Chang-ye Hui ◽  
Yan Guo ◽  
Jian Wu ◽  
Lisa Liu ◽  
Xue-qin Yang ◽  
...  

Cadmium (Cd) is carcinogenic to humans and can accumulate in the liver, kidneys, and bones. There is widespread presence of cadmium in the environment as a consequence of anthropogenic activities. It is important to detect cadmium in the environment to prevent further exposure to humans. Previous whole-cell biosensor designs were focused on single-sensing constructs but have had difficulty in distinguishing cadmium from other metal ions such as lead (Pb) and mercury (Hg). We developed a dual-sensing bacterial bioreporter system to detect bioavailable cadmium by employing CadC and CadR as separate metal sensory elements and eGFP and mCherry as fluorescent reporters in one genetic construct. The capability of this dual-sensing biosensor was proved to simultaneously detect bioavailable cadmium and its toxic effects using two sets of sensing systems while still maintaining similar specificity and sensitivity of respective signal-sensing biosensors. The productions of double-color fluorescence were directly proportional to the exposure concentration of cadmium, thereby serving as an effective quantitative biosensor to detect bioavailable cadmium. This novel dual-sensing biosensor was then validated to respond to Cd(II) spiked in environmental water samples. This is the first report of the development of a novel dual-sensing, whole-cell biosensor for simultaneous detection of bioavailable cadmium. The application of two biosensing modules provides versatile biosensing signals and improved performance that can make a significant impact on monitoring high concentration of bioavailable Cd(II) in environmental water to reduce human exposure to the harmful effects of cadmium.


RSC Advances ◽  
2017 ◽  
Vol 7 (47) ◽  
pp. 29302-29305 ◽  
Author(s):  
Pei-Hsuan Chen ◽  
Chieh Lin ◽  
Kai-Hong Guo ◽  
Yi-Chun Yeh

A engineered whole-cell biosensor is developed to generate output signals for the environmental copper analysis.


2002 ◽  
Vol 87 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Lars Hestbjerg Hansen ◽  
Frank Aarestrup ◽  
Søren Johannes Sørensen

2016 ◽  
Vol 239 ◽  
pp. 39-46 ◽  
Author(s):  
Maxime Toussaint ◽  
Cyril Bontemps ◽  
Arnaud Besserer ◽  
Laurence Hotel ◽  
Philippe Gérardin ◽  
...  

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