Swarm Intelligence in Cell Entry Exclusion Phenomena in Viruses and Plasmids: How to Exploit Intelligent Gene Vector Self-Scattering in Therapeutic Gene Delivery

2019 ◽  
pp. 325-343
Author(s):  
Oleg E. Tolmachov
Keyword(s):  
Gene Delivery ◽  
Swarm Intelligence ◽  
Cell Entry ◽  
Gene Vector ◽  
Therapeutic Gene

scholarly journals Destination Brain: the Past, Present, and Future of Therapeutic Gene Delivery

2017 ◽  
Vol 12 (1) ◽  
pp. 51-83 ◽  
Author(s):  
Chaitanya R. Joshi ◽  
Vinod Labhasetwar ◽  
Anuja Ghorpade
Keyword(s):  
Gene Delivery ◽  
Therapeutic Gene ◽  
The Past

Therapeutic gene delivery in human B-lymphoblastoid cells by engineered non-transforming infectious Epstein–Barr virus

1995 ◽  
Vol 1 (12) ◽  
pp. 1303-1308 ◽  
Author(s):  
Subrata Banerjee ◽  
Elizabeth Livanos ◽  
Jean-Michel H. Vos
Keyword(s):  
Gene Delivery ◽  
Epstein Barr Virus ◽  
Therapeutic Gene ◽  
Lymphoblastoid Cells ◽  
Barr Virus ◽  
Epstein Barr

Magnetic nanoparticles for targeted therapeutic gene delivery and magnetic-inducing heating on hepatoma

2014 ◽  
Vol 25 (34) ◽  
pp. 345101 ◽  
Author(s):  
Chenyan Yuan ◽  
Yanli An ◽  
Jia Zhang ◽  
Hongbo Li ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Gene Delivery ◽  
Therapeutic Gene ◽  
Targeted Therapeutic

Irradiation promotes Akt-targeting therapeutic gene delivery to the tumor vasculature

2007 ◽  
Vol 67 (4) ◽  
pp. 1155-1162 ◽  
Author(s):  
Pierre Sonveaux ◽  
Françoise Frérart ◽  
Caroline Bouzin ◽  
Agnès Brouet ◽  
Julie DeWever ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Tumor Vasculature ◽  
Therapeutic Gene

scholarly journals Regulation of Adenovirus Membrane Penetration by the Cytoplasmic Tail of Integrin β5

2000 ◽  
Vol 74 (6) ◽  
pp. 2731-2739 ◽  
Author(s):  
Kena Wang ◽  
Tinglu Guan ◽  
David A. Cheresh ◽  
Glen R. Nemerow
Keyword(s):  
Gene Delivery ◽  
Cytoplasmic Tail ◽  
Membrane Permeabilization ◽  
Cell Entry ◽  
Membrane Penetration ◽  
Wild Type ◽  
Endosomal Membrane ◽  
Cytoplasmic Vesicles ◽  
Thin Section Electron Microscopy ◽  
Section Electron

ABSTRACT Adenovirus (Ad) cell entry involves sequential interactions with host cell receptors that mediate attachment (CAR), internalization (αvβ3 and αvβ5), and penetration (αvβ5) of the endosomal membrane. These events allow the virus to deliver its genome to the nucleus. While integrins αvβ3 and αvβ5 both promote Ad internalization into cells, integrin αvβ5 selectively facilitates Ad-mediated membrane permeabilization and endosome rupture. In the experiments reported herein, we demonstrate that the intracellular domain of the integrin β5 subunit specifically regulates Ad-mediated membrane permeabilization and gene delivery. CS-1 melanoma cells expressing a truncated integrin β5 or a chimeric (β5-β3) cytoplasmic tail (CT) supported normal levels of Ad endocytosis but had reduced Ad-mediated gene delivery and membrane permeabilization relative to cells expressing a wild-type integrin β5. Thin-section electron microscopy revealed that virion particles were capable of being endocytosed into cells expressing a truncated β5CT, but they failed to escape cytoplasmic vesicles and translocate to the nucleus. Site-specific mutagenesis studies suggest that a C-terminal TVD motif in the β5CT plays a major role in Ad membrane penetration.

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