Cohesion is an important construct in understanding human-autonomy team dynamics and effectiveness, yet methods to adequately measure this construct are still being developed. Here we describe the initial steps of the development of a new human-autonomy team cohesion scale: item development, content validation, and preliminary scale item evaluation. The initial item pool was developed resulting in 134 items and underwent content validation with subject matter experts to reduce the item pool to 82. For the content validation, participants watched a set of video clips displaying examples of high and low human-autonomy team cohesion and rated the clips using the new human-autonomy team cohesion scale. Preliminary results revealed that our new human-autonomy team cohesion scale is a reliable and valid measure of cohesion in these contexts. Additionally, ratings for the high cohesion clips were somewhat higher than the low cohesion clips. Further analyses will determine if each of the items are contributing to the overall validity of the scale and will be further evaluated for possible removal.