Resolution-Improvement of Confocal Fluorescence Microscopy via Two Different Point Spread Functions

2020 ◽  
pp. 77-84
Author(s):  
Xuanhoi Hoang ◽  
Vannhu Le ◽  
MinhNghia Pham
Keyword(s):  
Fluorescence Microscopy ◽  
Confocal Fluorescence Microscopy ◽  
Point Spread Functions ◽  
Point Spread ◽  
Confocal Fluorescence ◽  
Resolution Improvement

Deconvolution in 3-D Microscopy: Applications and Limitations

1998 ◽  
Vol 4 (S2) ◽  
pp. 880-881
Author(s):  
P.J. Shaw
Keyword(s):  
Fluorescence Microscopy ◽  
Point Spread Function ◽  
Computational Procedure ◽  
Confocal Fluorescence Microscopy ◽  
Wide Field ◽  
Entire Field ◽  
Point Spread ◽  
Spread Function ◽  
Confocal Fluorescence

Many imaging processes, including both conventional wide-field and confocal fluorescence microscopy, can be described to a good approximation as linear and spatially-invariant. Linearity means that the image of an extended specimen is simply the sum of the images of the parts of the specimen - in the limit the specimen can be regarded as a collection of points, and its image is the sum of the images of the points - i.e. weighted instances of the imaging system's point spread function (psf). In the case of spatially invariant imaging the psf is the same over the entire field of imaging. Mathematically, the image is the convolution of the specimen with the system psf. In principle, this convolution can be reversed to remove degradation introduced by the imaging process, a computational procedure often called deconvolution or, more generally, restoration.

Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells

2019 ◽  
Vol 91 (17) ◽  
pp. 11129-11137 ◽  
Author(s):  
Aleksandar J. Krmpot ◽  
Stanko N. Nikolić ◽  
Sho Oasa ◽  
Dimitrios K. Papadopoulos ◽  
Marco Vitali ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Confocal Fluorescence Microscopy ◽  
Live Cells ◽  
Dynamic Processes ◽  
Microscopy Imaging ◽  
Confocal Fluorescence ◽  
Fast Dynamic

Choline and phosphatidylcholine fluorescent derivatives localization in carcinoma cells studied by laser scanning confocal fluorescence microscopy

2005 ◽  
Vol 41 (10) ◽  
pp. 1453-1459 ◽  
Author(s):  
Anna M. Villa ◽  
Elvira Caporizzo ◽  
Antonio Papagni ◽  
Luciano Miozzo ◽  
Paola Del Buttero ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Carcinoma Cells ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence ◽  
Fluorescent Derivatives

scholarly journals Analysis of Polyhydroxyalkanoates Granules in Haloferax mediterranei by Double-Fluorescence Staining with Nile Red and SYBR Green by Confocal Fluorescence Microscopy

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1582
Author(s):  
Verónica Cánovas ◽  
Salvador Garcia-Chumillas ◽  
Fuensanta Monzó ◽  
Lorena Simó-Cabrera ◽  
Carmen Fernández-Ayuso ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Culture Media ◽  
Nile Red ◽  
Sybr Green ◽  
Confocal Fluorescence Microscopy ◽  
Fluorescence Staining ◽  
Haloferax Mediterranei ◽  
Optimized Protocol ◽  
Confocal Fluorescence ◽  
Pha Production

Haloferaxmediterranei is a haloarchaeon of high interest in biotechnology because it produces and mobilizes intracellular polyhydroxyalkanoate (PHA) granules during growth under stress conditions (limitation of phosphorous in the culture media), among other interesting metabolites (enzymes, carotenoids, etc.). The capability of PHA production by microbes can be monitored with the use of staining-based methods. However, the staining of haloarchaea cells is a challenging task; firstly, due to the high ionic strength of the medium, which is inappropriate for most of dyes, and secondly, due to the low permeability of the haloarchaea S-layer to macromolecules. In this work, Haloferax mediterranei is used as a halophilic archaeon model to describe an optimized protocol for the visualization and analysis of intracellular PHA granules in living cells. The method is based on double-fluorescence staining using Nile red and SYBR Green by confocal fluorescence microscopy. Thanks to this method, the capability of PHA production by new haloarchaea isolates could be easily monitored.

Novel nontoxic mitochondrial probe for confocal fluorescence microscopy

2006 ◽  
Vol 11 (3) ◽  
pp. 034014 ◽  
Author(s):  
Silvia Versari ◽  
Anna Maria Villa ◽  
Alessandro Villa ◽  
Silvia Maria Doglia ◽  
Giorgio A. Pagani ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence
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