Cintia N. Parsza
◽
Diego L. Mengual Gómez
◽
Jorge Alejandro Simonin
◽
Mariano Nicolás Belaich
◽
Pablo Daniel Ghiringhelli
Background:
Baculoviruses are insect pathogens with important biotechnological applications that transcend
their use as biological controllers of agricultural pests. One species, Autographa californica multiple nucleopolhyedrovirus
(AcMNPV) has been extensively exploited as a molecular platform to produce recombinant proteins and as a delivery vector
for genes in mammals, because it can transduce a wide range of mammalian cells and tissues without replicating or
producing progeny.
Objective/Method:
To investigate if the budded virions of Anticarsia gemmatalis multiple nucleopolhyedrovirus
(AgMNPV) species has the same ability, the viral genome was modified by homologous recombination into susceptible
insect cells to integrate reporter genes and then it was evaluated on mammalian cell lines in comparative form with respect
to equivalent viruses derived from AcMNPV. Besides, the replicative capacity of AgMNPV´s virions in mammals was
determined.
Results:
The experiments carried out showed that the recombinant variant of AgMNPV transduces and support the
expression of delivered genes but not replicates in mammalian cells.
Conclusion:
Consequently, this insect pathogen is proposed as an alternative of non-infectious viruses in humans to explore
new approaches in gene therapy and other applications based on the use of mammalian cells.
Microfluidic immunoassay for detection of serological antibodies: A potential tool for rapid evaluation of immunity against SARS-CoV-2
