Chemically Driven Phase Separation in Black Lipid Membranes

1990 ◽  
pp. 241-248
Author(s):  
S. Mittler-Neher ◽  
J. Spinke ◽  
W. Knoll
Keyword(s):  
Phase Separation ◽  
Lipid Membranes ◽  
Black Lipid Membranes

Ca2+-induced phase separation in black lipid membranes and its effect on the transport of a hydrophobic ion

1985 ◽  
Vol 813 (2) ◽  
pp. 221-229 ◽  
Author(s):  
Alfred Miller ◽  
Günther Schmidt ◽  
Hansjörg Eibl ◽  
Wolfgang Knoll
Keyword(s):  
Phase Separation ◽  
Lipid Membranes ◽  
Black Lipid Membranes

Chemically driven phase separation in black lipid membranes and its coupling to membrane functions

1992 ◽  
Vol 210-211 ◽  
pp. 756-759 ◽  
Author(s):  
J. Spinke ◽  
R. Blankenburg ◽  
S. Förster ◽  
M. Schmidt ◽  
J. Zöller ◽  
...  
Keyword(s):  
Phase Separation ◽  
Lipid Membranes ◽  
Black Lipid Membranes

Observation of Concanavalin-A receptors on hydrated planar erythrocyte membrane film by in situ electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 608-609
Author(s):  
Neng-Bo He ◽  
S.W. Hui
Keyword(s):  
Lipid Bilayers ◽  
Erythrocyte Membrane ◽  
Lipid Membranes ◽  
Surface Film ◽  
Biological Membranes ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Black Lipid Membranes ◽  
Fine Mesh ◽  
Planar Films

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

scholarly journals The Use of Tethered Bilayer Lipid Membranes to Identify the Mechanisms of Antimicrobial Peptide Interactions with Lipid Bilayers

Antibiotics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Amani Alghalayini ◽  
Alvaro Garcia ◽  
Thomas Berry ◽  
Charles Cranfield
Keyword(s):  
New Zealand ◽  
Patch Clamp ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Antimicrobial Agents ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Black Lipid Membranes ◽  
Peptide Interactions ◽  
New Research

This review identifies the ways in which tethered bilayer lipid membranes (tBLMs) can be used for the identification of the actions of antimicrobials against lipid bilayers. Much of the new research in this area has originated, or included researchers from, the southern hemisphere, Australia and New Zealand in particular. More and more, tBLMs are replacing liposome release assays, black lipid membranes and patch-clamp electrophysiological techniques because they use fewer reagents, are able to obtain results far more quickly and can provide a uniformity of responses with fewer artefacts. In this work, we describe how tBLM technology can and has been used to identify the actions of numerous antimicrobial agents.

scholarly journals Dynamics of Phase Separation in Lipid Membranes

2010 ◽  
Vol 98 (3) ◽  
pp. 664a
Author(s):  
Brian Camley ◽  
Frank L.H. Brown
Keyword(s):  
Phase Separation ◽  
Lipid Membranes

Pump and displacement currents of reconstituted ATP synthase on black lipid membranes

1988 ◽  
Vol 104 (2) ◽  
pp. 179-191 ◽  
Author(s):  
B. Christensen ◽  
M. Gutweiler ◽  
E. Grell ◽  
N. Wagner ◽  
R. Pabst ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Lipid Membranes ◽  
Black Lipid Membranes ◽  
Displacement Currents

Black Lipid Membranes from Polymerizable Lipids

1982 ◽  
Vol 21 (S5) ◽  
pp. 869-880 ◽  
Author(s):  
Roland Benz ◽  
Werner Pra� ◽  
Helmut Ringsdorf
Keyword(s):  
Lipid Membranes ◽  
Black Lipid Membranes ◽  
Polymerizable Lipids

scholarly journals Correlated diffusion in lipid bilayers

2021 ◽  
Vol 118 (48) ◽  
pp. e2113202118
Author(s):  
Rafael L. Schoch ◽  
Frank L. H. Brown ◽  
Gilad Haran
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Lipid Membranes ◽  
Supported Lipid Bilayers ◽  
Single Molecule Tracking ◽  
Black Lipid Membranes ◽  
Molecular Tracking ◽  
Physical Materials ◽  
Multiple Molecules ◽  
Major Target

Lipid membranes are complex quasi–two-dimensional fluids, whose importance in biology and unique physical/materials properties have made them a major target for biophysical research. Recent single-molecule tracking experiments in membranes have caused some controversy, calling the venerable Saffman–Delbrück model into question and suggesting that, perhaps, current understanding of membrane hydrodynamics is imperfect. However, single-molecule tracking is not well suited to resolving the details of hydrodynamic flows; observations involving correlations between multiple molecules are superior for this purpose. Here dual-color molecular tracking with submillisecond time resolution and submicron spatial resolution is employed to reveal correlations in the Brownian motion of pairs of fluorescently labeled lipids in membranes. These correlations extend hundreds of nanometers in freely floating bilayers (black lipid membranes) but are severely suppressed in supported lipid bilayers. The measurements are consistent with hydrodynamic predictions based on an extended Saffman–Delbrück theory that explicitly accounts for the two-leaflet bilayer structure of lipid membranes.

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