Black Lipid Membranes from Polymerizable Lipids

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

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Ca2+-induced phase separation in black lipid membranes and its effect on the transport of a hydrophobic ion

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(85)90237-8 ◽

1985 ◽

Vol 813 (2) ◽

pp. 221-229 ◽

Cited By ~ 10

Author(s):

Alfred Miller ◽

Günther Schmidt ◽

Hansjörg Eibl ◽

Wolfgang Knoll

Keyword(s):

Phase Separation ◽

Lipid Membranes ◽

Black Lipid Membranes

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The Use of Tethered Bilayer Lipid Membranes to Identify the Mechanisms of Antimicrobial Peptide Interactions with Lipid Bilayers

Antibiotics ◽

10.3390/antibiotics8010012 ◽

2019 ◽

Vol 8 (1) ◽

pp. 12 ◽

Cited By ~ 13

Author(s):

Amani Alghalayini ◽

Alvaro Garcia ◽

Thomas Berry ◽

Charles Cranfield

Keyword(s):

New Zealand ◽

Patch Clamp ◽

Lipid Bilayers ◽

Lipid Membranes ◽

Antimicrobial Agents ◽

Bilayer Lipid Membranes ◽

Bilayer Lipid ◽

Black Lipid Membranes ◽

Peptide Interactions ◽

New Research

This review identifies the ways in which tethered bilayer lipid membranes (tBLMs) can be used for the identification of the actions of antimicrobials against lipid bilayers. Much of the new research in this area has originated, or included researchers from, the southern hemisphere, Australia and New Zealand in particular. More and more, tBLMs are replacing liposome release assays, black lipid membranes and patch-clamp electrophysiological techniques because they use fewer reagents, are able to obtain results far more quickly and can provide a uniformity of responses with fewer artefacts. In this work, we describe how tBLM technology can and has been used to identify the actions of numerous antimicrobial agents.

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Pump and displacement currents of reconstituted ATP synthase on black lipid membranes

The Journal of Membrane Biology ◽

10.1007/bf01870929 ◽

1988 ◽

Vol 104 (2) ◽

pp. 179-191 ◽

Cited By ~ 6

Author(s):

B. Christensen ◽

M. Gutweiler ◽

E. Grell ◽

N. Wagner ◽

R. Pabst ◽

...

Keyword(s):

Atp Synthase ◽

Lipid Membranes ◽

Black Lipid Membranes ◽

Displacement Currents

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The labilizing effect of minute glass particles on black lipid membranes and the stabilizing action of a lipophilic glucofuranose derivative

Agents and Actions ◽

10.1007/bf01965378 ◽

1971 ◽

Vol 2 (1) ◽

pp. 33-38 ◽

Cited By ~ 4

Author(s):

H. Majer

Keyword(s):

Lipid Membranes ◽

Black Lipid Membranes

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Chemically Driven Phase Separation in Black Lipid Membranes

Water and Ions in Biomolecular Systems ◽

10.1007/978-3-0348-7253-9_24 ◽

1990 ◽

pp. 241-248

Author(s):

S. Mittler-Neher ◽

J. Spinke ◽

W. Knoll

Keyword(s):

Phase Separation ◽

Lipid Membranes ◽

Black Lipid Membranes

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Correlated diffusion in lipid bilayers

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.2113202118 ◽

2021 ◽

Vol 118 (48) ◽

pp. e2113202118

Author(s):

Rafael L. Schoch ◽

Frank L. H. Brown ◽

Gilad Haran

Keyword(s):

Lipid Bilayers ◽

Single Molecule ◽

Lipid Membranes ◽

Supported Lipid Bilayers ◽

Single Molecule Tracking ◽

Black Lipid Membranes ◽

Molecular Tracking ◽

Physical Materials ◽

Multiple Molecules ◽

Major Target

Lipid membranes are complex quasi–two-dimensional fluids, whose importance in biology and unique physical/materials properties have made them a major target for biophysical research. Recent single-molecule tracking experiments in membranes have caused some controversy, calling the venerable Saffman–Delbrück model into question and suggesting that, perhaps, current understanding of membrane hydrodynamics is imperfect. However, single-molecule tracking is not well suited to resolving the details of hydrodynamic flows; observations involving correlations between multiple molecules are superior for this purpose. Here dual-color molecular tracking with submillisecond time resolution and submicron spatial resolution is employed to reveal correlations in the Brownian motion of pairs of fluorescently labeled lipids in membranes. These correlations extend hundreds of nanometers in freely floating bilayers (black lipid membranes) but are severely suppressed in supported lipid bilayers. The measurements are consistent with hydrodynamic predictions based on an extended Saffman–Delbrück theory that explicitly accounts for the two-leaflet bilayer structure of lipid membranes.

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