Nuclear Pore Complexes: Structural Changes as Monitored by Time-Lapse Atomic Force Microscopy

2010 ◽  
pp. 18-19
Author(s):  
Margit Pavelka ◽  
Jürgen Roth
Keyword(s):  
Atomic Force Microscopy ◽  
Structural Changes ◽  
Time Lapse ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Pore Complexes

Towards monitoring transport of single cargos across individual nuclear pore complexes by time-lapse atomic force microscopy

2010 ◽  
Vol 171 (2) ◽  
pp. 154-162 ◽  
Author(s):  
Ning-Ping Huang ◽  
Mike Stubenrauch ◽  
Joachim Köser ◽  
Nicole Taschner ◽  
Ueli Aebi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Time Lapse ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Pore Complexes

scholarly journals Quantification of Biomolecular Dynamics Inside Real and Synthetic Nuclear Pore Complexes Using Time-Resolved Atomic Force Microscopy

ACS Nano ◽  
2019 ◽  
Vol 13 (7) ◽  
pp. 7949-7956 ◽  
Author(s):  
George J. Stanley ◽  
Bernice Akpinar ◽  
Qi Shen ◽  
Patrick D. Ellis Fisher ◽  
C. Patrick Lusk ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Time Resolved ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biomolecular Dynamics ◽  
Pore Complexes

scholarly journals Atomic force microscopy and Raman spectra profile of blood components associated with exposure to cigarette smoking

RSC Advances ◽  
2020 ◽  
Vol 10 (20) ◽  
pp. 11971-11981
Author(s):  
Alexel J. Burgara-Estrella ◽  
Mónica A. Acosta-Elías ◽  
Osiris Álvarez-Bajo ◽  
Erika Silva-Campa ◽  
Aracely Angulo-Molina ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cigarette Smoking ◽  
Raman Spectra ◽  
Tobacco Smoke ◽  
Structural Changes ◽  
Cell Damage ◽  
Blood Components ◽  
Force Microscopy ◽  
Atomic Force

Tobacco smoke contains several compounds with oxidant and pro-oxidant properties with the capability of producing structural changes in biomolecules, as well as cell damage.

Domain Structure of a Unique Bacterial Red Light Photoreceptor as Revealed by Atomic Force Microscopy

2014 ◽  
Vol 1652 ◽  
Author(s):  
Blaire A. Sorenson ◽  
Daniel J. Westcott ◽  
Alexandra C. Sakols ◽  
J. Santoro Thomas ◽  
Perry Anderson ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Domain Structure ◽  
Structural Changes ◽  
Rhodopseudomonas Palustris ◽  
Red Light ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Biologically Relevant ◽  
Force Microscopy ◽  
Atomic Force

ABSTRACTBacteriophytochromes (BphPs) are red-light photoreceptors found in photosynthetic and nonphotosynthetic bacteria that have been recently engineered as infrared fluorescent tissue markers. Light-induced, global structural changes are proposed to originate within their covalently bound biliverdin chromophore and propagate through the protein. Classical BphPs undergo reversible photoconversion between spectrally distinct light absorbing states, red (Pr) and far-red (Pfr), respectively. RpBph3 (P3), from Rhodopseudomonas palustris, photoconverts between a Pr and a unique near-red (Pnr) light-absorbing state. Due to size and photosensitivity of BphPs, structures of the intact proteins have not been resolved by nuclear magnetic resonance and/or X-ray crystallography. Therefore, structural details about the light and dark-adapted structures of the intact BphPs are not well understood at the molecular level. We have utilized fluid cell atomic force microscopy (AFM) to investigate the domain structure of intact P3 in its light-adapted state (Pnr). By varying the concentration of the protein, deposition time, and the ionic strength of the buffer, the aggregation of P3 on a mica surface can be controlled and single dimers may be observed in a biologically relevant media. Domain resolution has been achieved for several orientations of the dimer on the surface. The structural dimensions of the dimer have been compared to a modeled BphP in its intact form generated using PyMOL software. AFM experiments are currently underway to analyze the dark-adapted state (Pr) of P3 in order to observe the anticipated structural changes. Ultimately, the goal is to use AFM and other surface analytical methods such as scanning tunneling microscopy and electron microscopy to gain new insight into the unique photochemistry of P3.

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