scholarly journals Atomic force microscopy and Raman spectra profile of blood components associated with exposure to cigarette smoking

RSC Advances ◽  
2020 ◽  
Vol 10 (20) ◽  
pp. 11971-11981
Author(s):  
Alexel J. Burgara-Estrella ◽  
Mónica A. Acosta-Elías ◽  
Osiris Álvarez-Bajo ◽  
Erika Silva-Campa ◽  
Aracely Angulo-Molina ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cigarette Smoking ◽  
Raman Spectra ◽  
Tobacco Smoke ◽  
Structural Changes ◽  
Cell Damage ◽  
Blood Components ◽  
Force Microscopy ◽  
Atomic Force

Tobacco smoke contains several compounds with oxidant and pro-oxidant properties with the capability of producing structural changes in biomolecules, as well as cell damage.

scholarly journals Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcos Penedo ◽  
Tetsuya Shirokawa ◽  
Mohammad Shahidul Alam ◽  
Keisuke Miyazawa ◽  
Takehiko Ichikawa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Membrane ◽  
Cell Biology ◽  
Cell Damage ◽  
Efficiency Analysis ◽  
Living Cells ◽  
Biomolecular Recognition ◽  
Cell Penetration ◽  
Force Microscopy ◽  
Atomic Force

AbstractOver the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.

Domain Structure of a Unique Bacterial Red Light Photoreceptor as Revealed by Atomic Force Microscopy

2014 ◽  
Vol 1652 ◽  
Author(s):  
Blaire A. Sorenson ◽  
Daniel J. Westcott ◽  
Alexandra C. Sakols ◽  
J. Santoro Thomas ◽  
Perry Anderson ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Domain Structure ◽  
Structural Changes ◽  
Rhodopseudomonas Palustris ◽  
Red Light ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Biologically Relevant ◽  
Force Microscopy ◽  
Atomic Force

ABSTRACTBacteriophytochromes (BphPs) are red-light photoreceptors found in photosynthetic and nonphotosynthetic bacteria that have been recently engineered as infrared fluorescent tissue markers. Light-induced, global structural changes are proposed to originate within their covalently bound biliverdin chromophore and propagate through the protein. Classical BphPs undergo reversible photoconversion between spectrally distinct light absorbing states, red (Pr) and far-red (Pfr), respectively. RpBph3 (P3), from Rhodopseudomonas palustris, photoconverts between a Pr and a unique near-red (Pnr) light-absorbing state. Due to size and photosensitivity of BphPs, structures of the intact proteins have not been resolved by nuclear magnetic resonance and/or X-ray crystallography. Therefore, structural details about the light and dark-adapted structures of the intact BphPs are not well understood at the molecular level. We have utilized fluid cell atomic force microscopy (AFM) to investigate the domain structure of intact P3 in its light-adapted state (Pnr). By varying the concentration of the protein, deposition time, and the ionic strength of the buffer, the aggregation of P3 on a mica surface can be controlled and single dimers may be observed in a biologically relevant media. Domain resolution has been achieved for several orientations of the dimer on the surface. The structural dimensions of the dimer have been compared to a modeled BphP in its intact form generated using PyMOL software. AFM experiments are currently underway to analyze the dark-adapted state (Pr) of P3 in order to observe the anticipated structural changes. Ultimately, the goal is to use AFM and other surface analytical methods such as scanning tunneling microscopy and electron microscopy to gain new insight into the unique photochemistry of P3.

XANES AND ATOMIC FORCE MICROSCOPY STUDIES ON PRE- AND POST-IRRADIATED SAPPHIRE

2008 ◽  
Vol 22 (30) ◽  
pp. 3007-3013 ◽  
Author(s):  
D. M. BHARDWAJ ◽  
D. C. JAIN ◽  
RAVI KUMAR ◽  
R. P. GUPTA ◽  
K. B. GARG
Keyword(s):  
Atomic Force Microscopy ◽  
Single Crystal ◽  
South African ◽  
Structural Changes ◽  
Force Microscopy ◽  
Atomic Force Microscopy Technique ◽  
Microscopy Technique ◽  
Atomic Force ◽  
Sapphire Single Crystal ◽  
Edge Features

XANES measurements at the Fe - K edge on natural South African sapphire single crystal (corundum) and an irradiated sample with fluence 1 × 1012 Ni 6+ ions/cm 2 are reported. Some decrease in intensity of pre-edge features (1s → 3d) and increase in intensity of 1s → 4p transition in Fe is observed with Ni fluence. Structural changes and modification on surface of irradiated sapphire with Ni 6+ ion have been observed by the atomic force microscopy technique and discussed in the term of defects.

scholarly journals Atomic force microscopy-based microrheology reveals significant differences in the viscoelastic response between malign and benign cell lines

Open Biology ◽  
2014 ◽  
Vol 4 (5) ◽  
pp. 140046 ◽  
Author(s):  
Jan Rother ◽  
Helen Nöding ◽  
Ingo Mey ◽  
Andreas Janshoff
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Cell Lines ◽  
Loss Tangent ◽  
Structural Changes ◽  
Metastatic Potential ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Independent Information

Mechanical phenotyping of cells by atomic force microscopy (AFM) was proposed as a novel tool in cancer cell research as cancer cells undergo massive structural changes, comprising remodelling of the cytoskeleton and changes of their adhesive properties. In this work, we focused on the mechanical properties of human breast cell lines with different metastatic potential by AFM-based microrheology experiments. Using this technique, we are not only able to quantify the mechanical properties of living cells in the context of malignancy, but we also obtain a descriptor, namely the loss tangent, which provides model-independent information about the metastatic potential of the cell line. Including also other cell lines from different organs shows that the loss tangent ( G″ / G′ ) increases generally with the metastatic potential from MCF-10A representing benign cells to highly malignant MDA-MB-231 cells.

scholarly journals Microinductor-Fused Atomic Force Microscopy Cantilevers for Dynamic Imaging and Multimodal Manipulation

2021 ◽  
Author(s):  
Harris Mousoulis ◽  
Xin Xu ◽  
Robert Wilson ◽  
Garrett Chado ◽  
Joseph Wahlquist ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Characterization ◽  
Structural Changes ◽  
Single Cells ◽  
Dynamic Imaging ◽  
Detection Sensitivity ◽  
Force Microscopy ◽  
Rapid Acquisition ◽  
Atomic Force ◽  
Resonance Frequencies

Recent advances in atomic force microscopy (AFM) imaging and force spectroscopy have demonstrated improvements in rapid acquisition of quantitative data for applications in materials science, surface characterization, and biology. However, conventional AFM technology is limited in detection sensitivity and the ability to excite at off-resonance frequencies restricting broad utility of the technology. Here we demonstrate new AFM cantilevers fabricated with a planar microcoil at the tip region, which can be used to generate or sense highly-localized magnetic fields. Torsion/bending actuation of the cantilevers is accomplished with simple experimental configurations, enabling quantitative and simultaneous mapping of both stiffness and friction at the sample surface with more than one order of magnitude improvement in compositional contrast. Our method is compatible with commercial AFM systems, allowing us to clearly resolve small stiffness and friction variations in copolymer and biological samples that were difficult to detect by conventional AFM methods. In combination with fluorescence microscopy, we also generated localized fields to selectively stimulate and monitor structural changes in viable cells with nm-scale detail. Hybrid AFM cantilevers may be useful to characterize a broad range of complex material surfaces, in addition to combined physical and chemical analyses of single cells and biological microenvironments.

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