Human Peripheral Blood Monocyte Derived Dendritic Cells Assay for the Detection and Characterization of Sensitizers

Author(s):  
Andreas Schepky ◽  
Hendrik Reuter ◽  
Jochen Kühnl ◽  
Pierre Aeby
Blood ◽  
1999 ◽  
Vol 93 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Katsuaki Sato ◽  
Hiroshi Kawasaki ◽  
Hitomi Nagayama ◽  
Ryo Serizawa ◽  
Junji Ikeda ◽  
...  

Abstract We examined the potential involvement of two CC chemokine receptors (CCRs), CCR-1 and CCR-3, in the functional activation of granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4)–generated human peripheral blood monocyte-derived immature dendritic cells (DCs). Flow cytometric analysis showed that CCR-1, CCR-3, CCR-5, and CXC chemokine receptor (CXCR)-4 were expressed on the cell surface of monocyte-derived DCs. Treatment with a monoclonal antibody (MoAb) to either CCR-1 or CCR-3 but not MoAbs to CCR-5 and CXCR-4 abolished chemotactic migration of monocyte-derived DCs. The DCs treated with either the anti–CCR-1 MoAb or anti–CCR-3 MoAb were less efficient than untreated DCs in proliferation of allogeneic T cells (TCs) and TC-derived secretion of interferon-γ (IFN-γ). The homotypic aggregation of DCs and heterotypic aggregation of DCs with TCs were suppressed by the anti–CCR-1 MoAb or anti–CCR-3 MoAb. These results indicate that CCR-1 and CCR-3 specifically regulate interaction of TCs and DCs in the process of antigen presentation.


Blood ◽  
1999 ◽  
Vol 93 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Katsuaki Sato ◽  
Hiroshi Kawasaki ◽  
Hitomi Nagayama ◽  
Ryo Serizawa ◽  
Junji Ikeda ◽  
...  

We examined the potential involvement of two CC chemokine receptors (CCRs), CCR-1 and CCR-3, in the functional activation of granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4)–generated human peripheral blood monocyte-derived immature dendritic cells (DCs). Flow cytometric analysis showed that CCR-1, CCR-3, CCR-5, and CXC chemokine receptor (CXCR)-4 were expressed on the cell surface of monocyte-derived DCs. Treatment with a monoclonal antibody (MoAb) to either CCR-1 or CCR-3 but not MoAbs to CCR-5 and CXCR-4 abolished chemotactic migration of monocyte-derived DCs. The DCs treated with either the anti–CCR-1 MoAb or anti–CCR-3 MoAb were less efficient than untreated DCs in proliferation of allogeneic T cells (TCs) and TC-derived secretion of interferon-γ (IFN-γ). The homotypic aggregation of DCs and heterotypic aggregation of DCs with TCs were suppressed by the anti–CCR-1 MoAb or anti–CCR-3 MoAb. These results indicate that CCR-1 and CCR-3 specifically regulate interaction of TCs and DCs in the process of antigen presentation.


2000 ◽  
Vol 199 (2) ◽  
pp. 115-125 ◽  
Author(s):  
Katsuaki Sato ◽  
Hitomi Nagayama ◽  
Makoto Enomoto ◽  
Kenji Tadokoro ◽  
Takeo Juji ◽  
...  

2000 ◽  
Vol 164 (5) ◽  
pp. 2285-2295 ◽  
Author(s):  
Katsuaki Sato ◽  
Hiroshi Kawasaki ◽  
Hitomi Nagayama ◽  
Makoto Enomoto ◽  
Chikao Morimoto ◽  
...  

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