A Preliminary Study of Image Analysis for Parasite Detection on Honey Bees

The functional characterization of MEMS devices is relevant today since it aims at verifying the behavior of these devices, as well as improving their design. In this regard, this study focused on the functional characterization of a MEMS microgripper prototype suitable in biomedical applications: the measurement of the angular displacement of the microgripper comb-drive is carried out by means of two novel automatic procedures, based on an image analysis method, SURF-based (Angular Displacement Measurement based on Speeded Up Robust Features, ADMSURF) and FFT-based (Angular Displacement Measurement based on Fast Fourier Transform, ADMFFT) method, respectively. Moreover, the measurement results are compared with a Semi-Automatic Method (SAM), to evaluate which of them is the most suitable for the functional characterization of the device. The curve fitting of the outcomes from SAM and ADMSURF, showed a quadratic trend in agreement with the analytical model. Moreover, the ADMSURF measurements below 1° are affected by an uncertainty of about 0.08° for voltages less than 14 V, confirming its suitability for microgripper characterization. It was also evaluated that the ADMFFT is more suitable for measurement of rotations greater than 1° (up to 30°), with a measurement uncertainty of 0.02°, at 95% of confidence level.

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Hygienic behaviour in honey bees in the UK: a preliminary study

Bee World ◽

10.1080/0005772x.2003.11099567 ◽

2003 ◽

Vol 84 (1) ◽

pp. 19-26 ◽

Cited By ~ 7

Author(s):

Ruth Waite ◽

Michael Brown ◽

Helen Thompson

Keyword(s):

Honey Bees ◽

Hygienic Behaviour ◽

Preliminary Study ◽

The Uk

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Three-dimensional visualization and characterization of cracks in geothermal reservoir rock using image analysis of reconstructed μCT images: A preliminary study

10.1063/1.4868764 ◽

2014 ◽

Cited By ~ 4

Author(s):

Fourier Dzar Eljabbar Latief ◽

Selly Feranie

Keyword(s):

Image Analysis ◽

Three Dimensional ◽

Geothermal Reservoir ◽

Reservoir Rock ◽

Preliminary Study

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A preliminary study and its application for the development of the quantitative evaluation method of developed fingerprints on porous surfaces using densitometric image analysis

Analytical Science and Technology ◽

10.5806/ast.2016.29.3.142 ◽

2016 ◽

Vol 29 (3) ◽

pp. 142-153 ◽

Cited By ~ 1

Author(s):

Jae-Hyun Cho ◽

Hyo-Won Kim ◽

Min-Sun Kim ◽

Sung-Woon Choi

Keyword(s):

Image Analysis ◽

Quantitative Evaluation ◽

Evaluation Method ◽

Porous Surfaces ◽

Preliminary Study

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Estimation of the Acetylcholinesterase activity of honey bees in Nepal

10.1101/2020.12.31.424928 ◽

2021 ◽

Author(s):

Shishir Pandey ◽

Shankar Gotame ◽

Sachin Sejuwal ◽

Basant Giri ◽

Susma Giri

Keyword(s):

Honey Bees ◽

Protein Concentration ◽

Specific Activity ◽

Environmental Pollutants ◽

Acetylcholinesterase Activity ◽

Preliminary Study ◽

Specific Activities ◽

Lowry Assay ◽

Autumn And Winter ◽

Bee Colonies

AbstractDecline in honey bee colonies possess a serious threat to biodiversity and agriculture. Prior detection of the stresses with the help of biomarkers and their management ensures honey bee’s survivability. Acetylcholinesterase (AChE) is a promising biomarker to monitor exposure of honey bees towards environmental pollutants. In this preliminary study, we measured AChE activity in forager honey bees collected from six districts of Nepal, Kathmandu, Bhaktapur, Lalitpur, Chitwan, Rupandehi and Pyuthan during autumn and winter seasons. We estimated AChE tissue and specific activities from bee’s heads using commercial kit based on Ellman assay and protein concentration using Lowry assay. In total, we collected 716 foragers belonging to A. cerana, A. mellifera and A. dorsata. A significant increase in all three parameters measured: AChE tissue activity, AChE specific activity and protein concentration was observed in winter samples. Both AChE tissue and specific activities were lower in A. mellifera compared to either A. cerana or A. dorsata. Protein concentration was higher in A. mellifera than in A. dorsata and lower than in A. cerana. We show correlation between both AChE tissue and specific activities and protein concentration across season and species and discuss possible factors contributing to the observations. Our results clearly indicate the presence of stress in the winter which is manifested through overexpression of the AChE. We recommend a detailed study to determine the factors accountable for the stresses for better management of honey bees in Nepal.

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