Molecular Detection of Cucumber Mosaic Virus and Other RNA Viruses Based on New Techniques

2010 ◽  
pp. 47-96 ◽  
Author(s):  
Jishuang Chen
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Molecular Detection ◽  
Rna Viruses ◽  
New Techniques

scholarly journals Effects of DICER-like proteins 2, 3 and 4 on cucumber mosaic virus and tobacco mosaic virus infections in salicylic acid-treated plants

2009 ◽  
Vol 90 (12) ◽  
pp. 3010-3014 ◽  
Author(s):  
Mathew G. Lewsey ◽  
John P. Carr
Keyword(s):  
Salicylic Acid ◽  
Tobacco Mosaic Virus ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Rna Viruses ◽  
Virus Infections ◽  
Defence Mechanisms ◽  
Triple Mutant ◽  
Positive Sense

Salicylic acid (SA)-mediated resistance and RNA silencing are both important plant antiviral defence mechanisms. To investigate overlap between these resistance phenomena, we examined the ability of mutant Arabidopsis thaliana plants lacking DICER-like (DCL) endoribonucleases 2, 3 and 4 to exhibit SA-induced defence. We found that in dcl2/3/4 triple mutant plants, treatment with exogenous SA stimulated resistance to two positive-sense RNA viruses: cucumber mosaic virus and tobacco mosaic virus. We conclude that DCLs 2, 3 and 4, which are the predominant DCL endoribonucleases involved in silencing of positive-sense RNA viruses, are not required for effective SA-induced resistance to these viruses. However, the findings do not exclude RNA silencing from making a contribution to SA-mediated resistance in wild-type plants.

scholarly journals Template-Independent Repair of the 3′ End of Cucumber Mosaic Virus Satellite RNA Controlled by RNAs 1 and 2 of Helper Virus

1998 ◽  
Vol 72 (6) ◽  
pp. 5061-5066 ◽  
Author(s):  
József Burgyán ◽  
Fernando García-Arenal
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Sequence Comparison ◽  
Rna Viruses ◽  
Helper Virus ◽  
Viral Rna ◽  
Genome Integrity ◽  
In Planta ◽  
Satellite Rna ◽  
Cucumber Mosaic Cucumovirus

ABSTRACT RNA viruses which do not have a poly(A) tail or a tRNA-like structure for the protection of their vulnerable 3′ termini may have developed a different strategy to maintain their genome integrity. We provide evidence that deletions of up to 7 nucleotides from the 3′ terminus of cucumber mosaic cucumovirus (CMV) satellite RNA (satRNA) were repaired in planta in the presence of the helper virus (HV) CMV. Sequence comparison of 3′-end-repaired satRNA progenies, and of satRNA and HV RNA, suggested that the repair was not dependent on a viral template. The 3′ end of CMV satRNA lacking the last three cytosines was not repaired in planta in the presence of tomato aspermy cucumovirus (TAV), although TAV is an efficient helper for the replication of CMV satRNA. With use of pseudorecombinants constructed by the interchange of RNAs 1 and 2 of TAV and CMV, evidence was provided that the 3′-end repair was controlled by RNAs 1 and 2 of CMV, which encode subunits of the viral RNA replicase. These results, and the observation of short repeated sequences close to the 3′ terminus of repaired molecules, suggest that the HV replicase maintains the integrity of the satRNA genome, playing a role analogous to that of cellular telomerases.

Screening of Capsicum Accessions for Resistance to Cucumber Mosaic Virus

1997 ◽  
Vol 65 (4) ◽  
pp. 769-776 ◽  
Author(s):  
Shinji Monma ◽  
Yoshiteru Sakata
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus

scholarly journals Six New Subgroup I Members of Japanese Cucumber Mosaic Virus as Determined by Nucleotide Sequence Analysis on RNA3's cDNAs.

1996 ◽  
Vol 62 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Piyasak CHAUMPLUK ◽  
Yukiko SASAKI ◽  
Naoko NAKAJIMA ◽  
Hideaki NAGANO ◽  
Ikuo NAKAMURA ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Nucleotide Sequence Analysis
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