Membrane Proteins in Phospholipid Bilayers: Structure Determination by Solid-State NMR

2013 ◽  
pp. 1463-1470
Author(s):  
Bibhuti B. Das ◽  
Stanley J. Opella
Keyword(s):  
Solid State ◽  
Membrane Proteins ◽  
Structure Determination ◽  
Solid State Nmr ◽  
Phospholipid Bilayers

Rotational diffusion of membrane proteins in aligned phospholipid bilayers by solid-state NMR spectroscopy

2006 ◽  
Vol 178 (1) ◽  
pp. 162-165 ◽  
Author(s):  
Sang Ho Park ◽  
Anthony A. Mrse ◽  
Alexander A. Nevzorov ◽  
Anna A. De Angelis ◽  
Stanley J. Opella
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Membrane Proteins ◽  
Solid State Nmr ◽  
Rotational Diffusion ◽  
Phospholipid Bilayers ◽  
Solid State Nmr Spectroscopy

Solid state NMR of membrane proteins: methods and applications

2021 ◽  
Author(s):  
Vivien Yeh ◽  
Boyan B. Bonev
Keyword(s):  
Solid State ◽  
High Resolution ◽  
Membrane Proteins ◽  
Solid State Nmr ◽  
Lipid Membrane ◽  
Cell Function ◽  
Membrane Lipid ◽  
Pharmacological Intervention ◽  
Nmr Methods ◽  
Informative Approach

Membranes of cells are active barriers, in which membrane proteins perform essential remodelling, transport and recognition functions that are vital to cells. Membrane proteins are key regulatory components of cells and represent essential targets for the modulation of cell function and pharmacological intervention. However, novel folds, low molarity and the need for lipid membrane support present serious challenges to the characterisation of their structure and interactions. We describe the use of solid state NMR as a versatile and informative approach for membrane and membrane protein studies, which uniquely provides information on structure, interactions and dynamics of membrane proteins. High resolution approaches are discussed in conjunction with applications of NMR methods to studies of membrane lipid and protein structure and interactions. Signal enhancement in high resolution NMR spectra through DNP is discussed as a tool for whole cell and interaction studies.

Solid-State NMR Structure Determination of Whole Anchoring Threads from the Blue Mussel Mytilus edulis

2012 ◽  
Vol 14 (1) ◽  
pp. 132-141 ◽  
Author(s):  
Alexandre A. Arnold ◽  
Frédéric Byette ◽  
Marc-Olivier Séguin-Heine ◽  
André LeBlanc ◽  
Lekha Sleno ◽  
...  
Keyword(s):  
Solid State ◽  
Mytilus Edulis ◽  
Structure Determination ◽  
Solid State Nmr ◽  
Blue Mussel ◽  
Nmr Structure ◽  
Nmr Structure Determination

A Proton-Detected 4D Solid-State NMR Experiment for Protein Structure Determination

ChemPhysChem ◽  
2011 ◽  
Vol 12 (5) ◽  
pp. 915-918 ◽  
Author(s):  
Matthias Huber ◽  
Sebastian Hiller ◽  
Paul Schanda ◽  
Matthias Ernst ◽  
Anja Böckmann ◽  
...  
Keyword(s):  
Protein Structure ◽  
Solid State ◽  
Structure Determination ◽  
Solid State Nmr ◽  
Protein Structure Determination
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