Evaluation of BOLD effects in the rat cortex

Purpose: This study aimed to characterize Blood oxygen level-dependent (BOLD) effects in 1H- MR spectra obtained during optogenetic activation of the rat forelimb cortex for the correction and estimation of accurate metabolite concentration changes. Methods : T2*-induced effects were characterized by linewidth changes and amplitude changes of water, NAA and tCr spectral peaks during the stimulation paradigm. Spectral linewidth-matching procedures were used to correct for the line-narrowing effect induced by BOLD. For an increased understanding of spectroscopic BOLD effects and the optimized way to correct them, a 1 Hz line-narrowing effect was also simulated on mouseproton MR spectrum1H-fMRS data acquired using STEAM acquisitions at 9.4T in rats (n=8) upon optogenetic stimulation of the primary somatosensory cortex were used. Data were analyzed with MATLAB routines and LCModel. Uncorrected and corrected 1H-MR spectra of simulated and in-vivo data were quantified and compared. BOLD-corrected difference spectra were also calculated and analyzed. Results: Significant mean increases in water and NAA peak heights (+ 1.1% and +4.5%, respectively) were found accompanied by decreased linewidths (-0.5 Hz and -2.8%) upon optogenetic stimulation. These estimates were used for further definition of an accurate line-broadening factor (lb). Usage of an erroneous lb introduced false-positive errors in metabolite concentration change estimates thereby altering the specificity of findings. Using different water scalings within LCModel, the water and metabolite BOLD contributions were separated. Conclusion : The linewidth-matching procedure using a precise lb factor remains the most performant approach for the accurate quantification of small (0.3 micromol/g) metabolic changes in 1H-fMRS studies. A simple and preliminary compartmentation of BOLD effects was proposed, which will require validation.

Determination of the Best Empiric Method to Quantify the Amplified Spontaneous Emission Threshold in Polymeric Active Waveguides

Amplified Spontaneous Emission (ASE) threshold represents a crucial parameter often used to establish if a material is a good candidate for applications to lasers. Even if the ASE properties of conjugated polymers have been widely investigated, the specific literature is characterized by several methods to determine the ASE threshold, making comparison among the obtained values impossible. We quantitatively compare 9 different methods employed in literature to determine the ASE threshold, in order to find out the best candidate to determine the most accurate estimate of it. The experiment has been performed on thin films of an homopolymer, a copolymer and a host:guest polymer blend, namely poly(9,9-dioctylfluorene) (PFO), poly(9,9-dioctylfluorene-cobenzothiadiazole) (F8BT) and F8BT:poly(3- hexylthiophene) (F8BT:rrP3HT), applying the Variable Pump Intensity (VPI) and the Variable Stripe Length (VSL) methods. We demonstrate that, among all the spectral features affected by the presence of ASE, the most sensitive is the spectral linewidth and that the best way to estimate the ASE threshold is to determine the excitation density at the beginning of the line narrowing. We also show that the methods most frequently used in literature always overestimate the threshold up to more than one order of magnitude.

Shpol'skii spectroscopy of metabolites of polycyclic aromatic hydrocarbons in primary alcohols at 77 K and 4.2 K

Line-narrowing effects of primary alcohols on the spectral features and fluorescence lifetimes of PAH metabolites is investigated for analytical purposes.

Surface Lattice Resonances in THz Metamaterials

Diffraction of light in periodic structures is observed in a variety of systems including atoms, solid state crystals, plasmonic structures, metamaterials, and photonic crystals. In metamaterials, lattice diffraction appears across microwave to optical frequencies due to collective Rayleigh scattering of periodically arranged structures. Light waves diffracted by these periodic structures can be trapped along the metamaterial surface resulting in the excitation of surface lattice resonances, which are mediated by the structural eigenmodes of the metamaterial cavity. This has brought about fascinating opportunities such as lattice-induced transparency, strong nearfield confinement, and resonant field enhancement and line-narrowing of metamaterial structural resonances through lowering of radiative losses. In this review, we describe the mechanisms and implications of metamaterial-engineered surface lattice resonances and lattice-enhanced field confinement in terahertz metamaterials. These universal properties of surface lattice resonances in metamaterials have significant implications for the design of resonant metamaterials, including ultrasensitive sensors, lasers, and slow-light devices across the electromagnetic spectrum.