To verify the applicability of RBF neural network PID control on speed regulating start control for hydro-viscous drive system, analyze the principle of RBF neural network PID control, the simulation model is established based on SIMULINK and the control characteristics are analyzed based on the AMESim/MATLAB co-simulation. The results show that RBF neural network PID control has a good self-correcting effect on speed regulating start of hydro-viscous; it can make right judgments according to the error and error rate and adjust the output speed towards opposite direction of error; meanwhile, it ensures the smoothness of output curve and avoids excessive mechanical impact. The results play a guiding role for control strategy selection of speed regulating start.