Study on the Fermentation Conditions and the Application in Feather Degradation of Keratinase Produced by Bacillus licheniformis

2013 ◽  
pp. 89-98
Author(s):  
Yu Li ◽  
Shuai Fan ◽  
Sheng Chen ◽  
Hao Er ◽  
Jianjie Du ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Feather Degradation ◽  
Fermentation Conditions

Fermentation conditions of serine/alkaline milk-clotting enzyme production by newly isolated Bacillus licheniformis BL312

2019 ◽  
Vol 69 (12) ◽  
pp. 1289-1300 ◽  
Author(s):  
Yao Zhang ◽  
Yongjun Xia ◽  
Phoency F.-H. Lai ◽  
Xiaofeng Liu ◽  
Zhiqiang Xiong ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Enzyme Production ◽  
Fermentation Conditions ◽  
Milk Clotting ◽  
Milk Clotting Enzyme

scholarly journals Screening and Characterisation of Keratin-Degrading Bacillus sp. from Feather Waste

2019 ◽  
pp. 1-12
Author(s):  
M. T. Dada ◽  
S. M. Wakil
Keyword(s):  
Bacillus Subtilis ◽  
Proteolytic Activity ◽  
Bacillus Licheniformis ◽  
Basal Medium ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Feather Degradation ◽  
Feather Waste ◽  
Degrading Bacteria ◽  
Significant Difference

Aim: This study focuses on the screening and characterisation of keratin-degrading Bacillus species from feather waste. Methods: Nine bacteria were isolated from feather waste obtained from a poultry layout at Egbeda local government secretariat, Ibadan, Nigeria. These bacteria were grown in basal medium with feather as primary source of carbon, nitrogen, sulfur and energy. Feather degrading bacteria were screened for both proteolytic activity and keratin degradation on skimmed milk agar and keratin azure medium respectively. They were also screened for their ability to degrade other keratin substrates such as hair and nail. Results: Three of the isolates with higher feather degradation levels also showed high proteolytic activity and release of azure dye. They were selected and identified phenotypically and genotypically using 16S rRNA sequencing as Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53. The bacteria were capable of degrading other keratin-containing substrates such as nail and hair. Bacillus subtilis-K50 and Bacillus licheniformis-K51 showed significant difference (P) in degradation among the three different keratin sources used yielding higher degradation with feather as keratin source with respective optical densities of 0.07 and 0.11 followed by hair and least in nails with optical densities of 0.05 and 0.07 respectively. Highest degradation of all the three keratin substrates was observed in Bacillus licheniformis-K51. Conclusion: The three isolated bacteria possess the ability to degrade keratin and utilize feather as keratin substrate. As a result, these can be considered as potential candidates for degradation and utilization of feather keratin.

Influence of medium components and fermentation conditions on the production of bacteriocin(s) by Bacillus licheniformis AnBa9

2009 ◽  
Vol 100 (2) ◽  
pp. 872-877 ◽  
Author(s):  
Thangamani Anthony ◽  
Thangamani Rajesh ◽  
Nagarajan Kayalvizhi ◽  
Paramasamy Gunasekaran
Keyword(s):  
Bacillus Licheniformis ◽  
Fermentation Conditions ◽  
Medium Components

Keratinolytic potential ofBacillus licheniformisRG1: structural and biochemical mechanism of feather degradation

2005 ◽  
Vol 51 (3) ◽  
pp. 191-196 ◽  
Author(s):  
Priya Ramnani ◽  
Rajni Singh ◽  
Rani Gupta
Keyword(s):  
Bacillus Licheniformis ◽  
Bacterial Adhesion ◽  
Degradation Process ◽  
Bacterial Cells ◽  
Disulfide Bridges ◽  
Feather Degradation ◽  
Keratinolytic Activity ◽  
Disulfide Reductase ◽  
Live Bacterial Cells ◽  
Disulfide Reductases

Keratinolytic Bacillus licheniformis RG1 was used to study the mechanism of keratinolysis. Scanning electron microscopy studies revealed that bacterial cells grew closely adhered to the barbules of feathers, completely degrading them within 24 h. Biochemical studies indicated that the Bacillus strain produced an extracellular protease, which had keratinolytic potential. The extracellular keratinolytic activity (425 U) was synergistically enhanced by the addition of intracellular disulfide reductases (1712 U). However, these enzymes alone (keratinase and disulfide reductase), without live bacterial cells, failed to degrade the feather. Complete feather degradation was obtained only when living bacterial cells were present, emphasizing that bacterial adhesion plays a key role during the degradation process. The bacterial cells probably provide a continuous supply of reductant to break disulfide bridges. In addition, sulfite detected in the extracellular broth during feather degradation indicated that sulfitolysis may also play a role in feather degradation by the bacterium.Key words: Bacillus licheniformis, disulfide reductase, keratinase, sulfitolysis.

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