Studies on the Cholinergic Differentiation Factor for Cultured Sympathetic Neurons

1985 ◽  
pp. 13-17
Author(s):  
K. Fukada
Keyword(s):  
Sympathetic Neurons ◽  
Differentiation Factor ◽  
Cultured Sympathetic Neurons

scholarly journals Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons

2006 ◽  
Vol 51 (3) ◽  
pp. 397-413 ◽  
Author(s):  
Patricia C. Brum ◽  
Carl M. Hurt ◽  
Olga G. Shcherbakova ◽  
Brian Kobilka ◽  
Timothy Angelotti
Keyword(s):  
Adrenergic Receptor ◽  
Sympathetic Neurons ◽  
Receptor Subtypes ◽  
Cultured Sympathetic Neurons ◽  
And Function

Tau confers drug stability but not cold stability to microtubules in living cells

1994 ◽  
Vol 107 (1) ◽  
pp. 135-143 ◽  
Author(s):  
P.W. Baas ◽  
T.P. Pienkowski ◽  
K.A. Cimbalnik ◽  
K. Toyama ◽  
S. Bakalis ◽  
...  
Keyword(s):  
Sympathetic Neurons ◽  
Drug Stability ◽  
Living Cells ◽  
Microtubule Depolymerization ◽  
Cell Biol ◽  
Cold Stability ◽  
The Stability ◽  
Cultured Sympathetic Neurons ◽  
Very High

We previously defined two classes of microtubule polymer in the axons of cultured sympathetic neurons that differ in their sensitivity to nocodazole by roughly 35-fold (Baas and Black (1990) J. Cell Biol. 111, 495–509). Here we demonstrate that virtually all of the microtubule polymer in these axons, including the drug-labile polymer, is stable to cold. What factors account for the unique stability properties of axonal microtubules? In the present study, we have focused on the role of tau, a microtubule-associated protein that is highly enriched in the axon, in determining the stability of microtubules to nocodazole and/or cold in living cells. We used a baculovirus vector to express very high levels of tau in insect ovarian Sf9 cells. The cells respond by extending processes that contain dense bundles of microtubules (Knops et al. (1991) J. Cell Biol. 114, 725–734). Cells induced to express tau were treated with either cold or 2 micrograms/ml nocodazole for times ranging from 5 minutes to 6 hours. The results with each treatment were very different from one another. Virtually all of the polymer was depolymerized within the first 30 minutes in cold, while little or no microtubule depolymerization was detected even after 6 hours in nocodazole. Based on these results, we conclude that tau is almost certainly a factor in conferring drug stability to axonal microtubules, but that factors other than or in addition to tau are required to confer cold stability.

scholarly journals Acute Inactivation of Tau Has No Effect on Dynamics of Microtubules in Growing Axons of Cultured Sympathetic Neurons

1998 ◽  
Vol 18 (21) ◽  
pp. 8660-8673 ◽  
Author(s):  
Irina Tint ◽  
Theresa Slaughter ◽  
Itzhak Fischer ◽  
Mark M. Black
Keyword(s):  
Sympathetic Neurons ◽  
Cultured Sympathetic Neurons

scholarly journals Glia Induce Dendritic Growth in Cultured Sympathetic Neurons by Modulating the Balance between Bone Morphogenetic Proteins (BMPs) and BMP Antagonists

2002 ◽  
Vol 22 (23) ◽  
pp. 10377-10387 ◽  
Author(s):  
Pamela J. Lein ◽  
Hiroko Nagasawa Beck ◽  
Vidya Chandrasekaran ◽  
Patrick J. Gallagher ◽  
Hui-Ling Chen ◽  
...  
Keyword(s):  
Bone Morphogenetic Proteins ◽  
Dendritic Growth ◽  
Sympathetic Neurons ◽  
Bmp Antagonists ◽  
Cultured Sympathetic Neurons

Formation of inositol trisphosphate by muscarinic agents does not stimulate transmitter release in cultured sympathetic neurons

1988 ◽  
Vol 90 (1-2) ◽  
pp. 234-238 ◽  
Author(s):  
Sanjiv V. Bhave ◽  
Ravindra K. Malhotra ◽  
Taruna D. Wakade ◽  
Arun R. Wakade
Keyword(s):  
Transmitter Release ◽  
Sympathetic Neurons ◽  
Inositol Trisphosphate ◽  
Cultured Sympathetic Neurons ◽  
Muscarinic Agents
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