Structure of cell coats during initial stages of synapse formation on isolated cultured sympathetic neurons

1978 ◽  
Vol 7 (6) ◽  
pp. 679-691 ◽  
Author(s):  
Rosemary P. Rees
Keyword(s):  
Synapse Formation ◽  
Sympathetic Neurons ◽  
Cultured Sympathetic Neurons

scholarly journals Growth cones of cultured sympathetic neurons contain adrenergic vesicles.

1978 ◽  
Vol 78 (1) ◽  
pp. R8 ◽  
Author(s):  
S C Landis
Keyword(s):  
Sensory Neurons ◽  
Target Cell ◽  
Synaptic Vesicles ◽  
Dorsal Root ◽  
Synapse Formation ◽  
Sympathetic Neurons ◽  
Growth Cones ◽  
Cultured Neurons ◽  
Granular Vesicles ◽  
Cultured Sympathetic Neurons

The growth cones of dissociated rat sympathetic neurons developing in culture were fixed with potassium permanganate to visualize vesicular stores of norepinephrine through the formation of granular precipitates. It was found that growth cones contain numerous small granular vesicles (SGV) 40-60 nm in diameter. The majority of the SGV was present in the varicosity of the growth cone but SGV also occurred in filopodia. The SGV appeared in clusters or scattered throughout the varicosity. Treatment of the cultured neurons, before fixation, with reserpine, which depletes catecholamine stores by blocking uptake into vesicles, resulted in the presence of small clear vesicles. In contrast, growth cones of nonadrenergic sensory neurons dissociated from dorsal root ganglia and fixed with permanganate lacked SGV and possessed small clear vesicles. These observations indicate that the growth cones of cultured sympathetic neurons contain norepinephrine, suggest that the norepinephrine is stored in synaptic vesicles, and raise the question whether this transmitter plays a role in early axon-target cell interactions during synapse formation.

scholarly journals Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons

2006 ◽  
Vol 51 (3) ◽  
pp. 397-413 ◽  
Author(s):  
Patricia C. Brum ◽  
Carl M. Hurt ◽  
Olga G. Shcherbakova ◽  
Brian Kobilka ◽  
Timothy Angelotti
Keyword(s):  
Adrenergic Receptor ◽  
Sympathetic Neurons ◽  
Receptor Subtypes ◽  
Cultured Sympathetic Neurons ◽  
And Function

Vasoactive intestinal polypeptide presynaptically enhances the synaptic transmission in cultured sympathetic neurons

1992 ◽  
Vol 579 (2) ◽  
pp. 204-210 ◽  
Author(s):  
Mie Takahashi ◽  
Masaharu Ogawa ◽  
Hitoshi Ohta ◽  
Hisao Ikeda
Keyword(s):  
Synaptic Transmission ◽  
Vasoactive Intestinal Polypeptide ◽  
Sympathetic Neurons ◽  
Cultured Sympathetic Neurons ◽  
Intestinal Polypeptide

scholarly journals Induction of Cholinergic Function in Cultured Sympathetic Neurons by Periosteal Cells: Cellular Mechanisms

2001 ◽  
Vol 235 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Stephen E. Asmus ◽  
Hua Tian ◽  
Story C. Landis
Keyword(s):  
Sympathetic Neurons ◽  
Cellular Mechanisms ◽  
Cholinergic Function ◽  
Periosteal Cells ◽  
Cultured Sympathetic Neurons

Tau confers drug stability but not cold stability to microtubules in living cells

1994 ◽  
Vol 107 (1) ◽  
pp. 135-143 ◽  
Author(s):  
P.W. Baas ◽  
T.P. Pienkowski ◽  
K.A. Cimbalnik ◽  
K. Toyama ◽  
S. Bakalis ◽  
...  
Keyword(s):  
Sympathetic Neurons ◽  
Drug Stability ◽  
Living Cells ◽  
Microtubule Depolymerization ◽  
Cell Biol ◽  
Cold Stability ◽  
The Stability ◽  
Cultured Sympathetic Neurons ◽  
Very High

We previously defined two classes of microtubule polymer in the axons of cultured sympathetic neurons that differ in their sensitivity to nocodazole by roughly 35-fold (Baas and Black (1990) J. Cell Biol. 111, 495–509). Here we demonstrate that virtually all of the microtubule polymer in these axons, including the drug-labile polymer, is stable to cold. What factors account for the unique stability properties of axonal microtubules? In the present study, we have focused on the role of tau, a microtubule-associated protein that is highly enriched in the axon, in determining the stability of microtubules to nocodazole and/or cold in living cells. We used a baculovirus vector to express very high levels of tau in insect ovarian Sf9 cells. The cells respond by extending processes that contain dense bundles of microtubules (Knops et al. (1991) J. Cell Biol. 114, 725–734). Cells induced to express tau were treated with either cold or 2 micrograms/ml nocodazole for times ranging from 5 minutes to 6 hours. The results with each treatment were very different from one another. Virtually all of the polymer was depolymerized within the first 30 minutes in cold, while little or no microtubule depolymerization was detected even after 6 hours in nocodazole. Based on these results, we conclude that tau is almost certainly a factor in conferring drug stability to axonal microtubules, but that factors other than or in addition to tau are required to confer cold stability.

scholarly journals Acute Inactivation of Tau Has No Effect on Dynamics of Microtubules in Growing Axons of Cultured Sympathetic Neurons

1998 ◽  
Vol 18 (21) ◽  
pp. 8660-8673 ◽  
Author(s):  
Irina Tint ◽  
Theresa Slaughter ◽  
Itzhak Fischer ◽  
Mark M. Black
Keyword(s):  
Sympathetic Neurons ◽  
Cultured Sympathetic Neurons

scholarly journals Glia Induce Dendritic Growth in Cultured Sympathetic Neurons by Modulating the Balance between Bone Morphogenetic Proteins (BMPs) and BMP Antagonists

2002 ◽  
Vol 22 (23) ◽  
pp. 10377-10387 ◽  
Author(s):  
Pamela J. Lein ◽  
Hiroko Nagasawa Beck ◽  
Vidya Chandrasekaran ◽  
Patrick J. Gallagher ◽  
Hui-Ling Chen ◽  
...  
Keyword(s):  
Bone Morphogenetic Proteins ◽  
Dendritic Growth ◽  
Sympathetic Neurons ◽  
Bmp Antagonists ◽  
Cultured Sympathetic Neurons

scholarly journals Cultured sympathetic neurons synthesize and release the cytokine interleukin 1 beta.

1992 ◽  
Vol 89 (21) ◽  
pp. 10440-10443 ◽  
Author(s):  
M. Freidin ◽  
M. V. Bennett ◽  
J. A. Kessler
Keyword(s):  
Sympathetic Neurons ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Cultured Sympathetic Neurons

Formation of inositol trisphosphate by muscarinic agents does not stimulate transmitter release in cultured sympathetic neurons

1988 ◽  
Vol 90 (1-2) ◽  
pp. 234-238 ◽  
Author(s):  
Sanjiv V. Bhave ◽  
Ravindra K. Malhotra ◽  
Taruna D. Wakade ◽  
Arun R. Wakade
Keyword(s):  
Transmitter Release ◽  
Sympathetic Neurons ◽  
Inositol Trisphosphate ◽  
Cultured Sympathetic Neurons ◽  
Muscarinic Agents
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