Effect of Nifedipine on DNA Synthesis and Cytosolic Free Calcium Concentration Stimulated by Growth Factors: A Study Using Cultured Rat Aortic Smooth-Muscle Cells

1989 ◽  
pp. 74-81
Author(s):  
Y. Ouchi ◽  
J. Hirosumi ◽  
M. Watanabe ◽  
J. Kusunoki ◽  
H. Orimo
Keyword(s):  
Smooth Muscle ◽  
Growth Factors ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Muscle Cells ◽  
Free Calcium ◽  
Cytosolic Free Calcium ◽  
Aortic Smooth Muscle ◽  
Free Calcium Concentration ◽  
Cytosolic Free Calcium Concentration

Role of smooth muscle cells on endothelial cell cytosolic free calcium in porcine coronary arteries

2001 ◽  
Vol 281 (3) ◽  
pp. H1156-H1162 ◽  
Author(s):  
Stéphane Budel ◽  
Alexander Schuster ◽  
Nikos Stergiopoulos ◽  
Jean-Jacques Meister ◽  
Jean-Louis Bény
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Coronary Arteries ◽  
Calcium Concentration ◽  
Muscle Cells ◽  
Free Calcium ◽  
Cytosolic Free Calcium ◽  
Free Calcium Concentration ◽  
Cytosolic Free Calcium Concentration

We tested the hypothesis that the cytosolic free calcium concentration in endothelial cells is under the influence of the smooth muscle cells in the coronary circulation. In the left descending branch of porcine coronary arteries, cytosolic free calcium concentration ([Ca2+]i) was estimated by determining the fluorescence ratio of two calcium probes, fluo 4 and fura red, in smooth muscle and endothelial cells using confocal microscopy. Acetylcholine and potassium, which act directly on smooth muscle cells to increase [Ca2+]i, were found to indirectly elevate [Ca2+]i in endothelial cells; in primary cultures of endothelial cells, neither stimulus affected [Ca2+]i, yet substance P increased the fluorescence ratio twofold. In response to acetylcholine and potassium, isometric tension developed by arterial strips with intact endothelium was attenuated by up to 22% ( P < 0.05) compared with strips without endothelium. These findings suggest that stimuli that increase smooth muscle [Ca2+]i can indirectly influence endothelial cell function in porcine coronary arteries. Such a pathway for negative feedback can moderate vasoconstriction and diminish the potential for vasospasm in the coronary circulation.

Vasoconstriction: a new activity for platelet-derived growth factor

Science ◽  
1986 ◽  
Vol 232 (4746) ◽  
pp. 87-90 ◽  
Author(s):  
BC Berk ◽  
RW Alexander ◽  
TA Brock ◽  
MA Gimbrone ◽  
RC Webb
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Rat Aorta ◽  
Platelet Derived Growth Factor ◽  
Free Calcium ◽  
Free Calcium Concentration ◽  
Cytosolic Free Calcium Concentration

Platelet-derived growth factor (PDGF) is a potent mitogen for vascular smooth muscle cells that has been implicated in the pathogenesis of atherosclerosis. The potential role of PDGF in the altered vasoreactivity of atherosclerotic vessels has been studied through an examination of its effects on contractility in the rat aorta. PDGF caused a concentration-dependent contraction of aortic strips and was significantly more potent on a molar basis than the classic vasoconstrictor peptide angiotensin II. Furthermore, PDGF increased the cytosolic free calcium concentration in cultured rat aortic smooth muscle cells. These observations suggest a new biological activity for PDGF that may contribute to the enhanced vasoreactivity of certain atherosclerotic vessels.

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