Simple Analysis of ESR Spectra of Spin-Labelled Macromolecules with Account of Fast Anisotropic Rotation of a Spin-Label and Slow Diffusion Rotation of a Macromolecule

1979 ◽  
pp. 521-521 ◽  
Author(s):  
I. V. Dudich ◽  
V. P. Timofeev ◽  
M. V. Volkenstein
Keyword(s):  
Spin Label ◽  
Esr Spectra ◽  
Simple Analysis ◽  
Slow Diffusion

Spin Label Study of Apomembranes and Purple Membranes

1993 ◽  
Vol 48 (5-6) ◽  
pp. 500-503
Author(s):  
Tzvetana R. Lazarova ◽  
Maya Y. Velitchkova
Keyword(s):  
Fatty Acids ◽  
Lipid Bilayer ◽  
Spin Label ◽  
Hyperfine Splitting ◽  
Esr Spectra ◽  
Spin Labels ◽  
Label Study ◽  
Splitting Parameter ◽  
Induced Changes ◽  
Purple Membranes

Abstract Three spin-labelled fatty acids were used to detect the dynamics of lipid bilayer of apomem branes and purple membranes. It was found that ESR spectra of spin labels bound to apo­ membranes showed a temperature-induced changes rather similar to those seen with purple membranes. At the same time, however, the values of hyperfine splitting parameter 2Tm were lower as compared to purple membranes. The results pointed out that the removal of the retinal from purple membranes affects the dynamics of lipid bilayer and apomembranes were more rigid structure than those of purple membranes.

Axial isomer and ESR spectra of the steroid spin label, 3-doxyl-5α-cholestane

1976 ◽  
Vol 24 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Thomas B Marriott ◽  
Shui Pong Van ◽  
O.Hayes Griffith
Keyword(s):  
Spin Label ◽  
Esr Spectra

scholarly journals Phase behaviour of DPPC/Lyso-PPC mixtures by spin-label ESR and spectrophotometry

2008 ◽  
Vol 22 (2-3) ◽  
pp. 153-163 ◽  
Author(s):  
Manuela Pantusa ◽  
Luigi Sportelli ◽  
Rosa Bartucci
Keyword(s):  
Spin Label ◽  
Acyl Chain ◽  
Fluid Phase ◽  
Esr Spectra ◽  
Phase Behaviour ◽  
Melting Transition ◽  
Micellar Aggregates ◽  
Lipid Chain ◽  
Main Transition ◽  
Gel Phase

The influence of lysopalmitoylphosphatidylcholine (Lyso-PPC) on lamellar dispersions of dipalmitoylphosphatidylcholine (DPPC) has been investigated by spectrophotometry and electron spin resonance (ESR) of spin-labelled phosphatidylcholine at the C-5 or at the C-16 positions in thesn-2 acyl chain (5- and 16-PCSL). On increasing the concentration of Lyso-PPC up to 10 mol%, the spectral anisotropy of 5-PCSL is slightly reduced whereas that of 16-PCSL is markedly increased. This effect of the lysolipid on the DPPC lipid chain mobility is evident from 10 to 50 mol% at low temperature in the gel phase, disappears in the fluid phase, is associated with the lack of the pre-transition in the mixed lipid dispersions and it is interpreted as interdigitation of the DPPC lipid chains. At intermediate concentrations and for temperatures in the gel phase, the ESR spectra of both labels in DPPC and Lyso-PPC mixtures are the superposition of an anisotropic lamellar component and of an isotropic micellar signal, suggesting the coexistence of lamellar and micellar aggregates. Chain interdigitation and lamellar-micellar coexistence are not evident for temperature above the melting transition temperature. At high Lyso-PPC content (≥60 mol%) the mixed lipid dispersions reach the optical clarity, the main transition is no more detected and spin-label ESR spectra typical of micellar dispersions are detected in the whole temperature range investigated.

Spin Label Studies of Erythrocytes during Storage of Blood

1989 ◽  
Vol 44 (9-10) ◽  
pp. 824-828 ◽  
Author(s):  
G. P. Pessina ◽  
L. Ciccoli ◽  
M. P. Picchi ◽  
G. Fanetti
Keyword(s):  
Human Erythrocyte ◽  
Spin Label ◽  
Room Temperature ◽  
Esr Spectra ◽  
Erythrocyte Membranes ◽  
Artificial Ageing ◽  
Human Erythrocyte Membrane ◽  
Signal Decay ◽  
Human Erythrocyte Membranes ◽  
Protein Free Medium

The kinetic behavior of the spin label MAL-6 in the interaction with differently aged human erythrocyte membranes was evaluated by monitoring the rate of disappearance of the room temperature ESR signal due to the MAL-6 spin label added to blood after storage at 4 °C or after incubation of red cells at 37 °C in a protein-free medium. After 35 days of blood storage or 60 h of erythrocytes incubation at 37 °C the decrease of the intensity of the MAL-6 ESR spectra in respect to control samples is markedly enhanced and the correspondent kinetic constants significantly increase. Signal decay of MAL-6 is a further proof that during storage of blood under blood bank conditions or during an artificial ageing of erythrocytes at 37 °C, profound modifications occur in the human erythrocyte membrane.

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