Determination of Clavibacter Michiganensis subsp. Insidiosus Infection in Lucerne Seeds by Immunofluorescence Staining and Direct Isolation Using Selective Medium

1997 ◽  
pp. 395-397
Author(s):  
J. Németh ◽  
A. N. Kovács
Keyword(s):  
Selective Medium ◽  
Immunofluorescence Staining ◽  
Clavibacter Michiganensis ◽  
Clavibacter Michiganensis Subsp

scholarly journals Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 291-293
Author(s):  
B. Kokošková ◽  
R. Jeřabková
Keyword(s):  
Monoclonal Antibodies ◽  
Metabolic Activity ◽  
Species Level ◽  
Colony Morphology ◽  
Clavibacter Michiganensis ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Atypical Cells ◽  
Das Elisa

Clavibacter michiganensis subsp. sepedonicus (Cms), causing ring rot of potato, is a quarantine bacterium. According to colony morphology, Cms occurs mostly as fluidal (smooth), but sometime as intermediate (rough, i.e. less fluidal) variants, too. Commercial monoclonal antibodies (Agdia, USA) were used for determination of 40 Cms strains representing both forms. All Cms strains were reliably identified by IFAS, but atypical cells were sometime recorded in population of intermediate strains. The fluidal Cms strains were more reliably identified using DAS-ELISA and the Biolog GP MicroPlate System<sup>TM</sup> than intermediate strains. The intermediate Cms strains had decreased metabolic activity compared with fluidal strains and that is why they were identified only to the genera or to the species level or not identified. The differences among fluidal and intermediate Cms strains were recorded also in bioassay on eggplants. The intermediate Cms strains caused atypical or no symptoms with comparison to fluidal strains.

scholarly journals Sensitivity and specificity of monoclonal antibody Mn-Cs1 for detection and determination of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato

2012 ◽  
Vol 38 (No. 4) ◽  
pp. 117-124 ◽  
Author(s):  
I. Pánková ◽  
B. Kokošková
Keyword(s):  
Monoclonal Antibody ◽  
Sensitivity And Specificity ◽  
Extracellular Polysaccharides ◽  
Bacterial Strains ◽  
Clavibacter Michiganensis ◽  
Bacterial Ring Rot ◽  
Clavibacter Michiganensis Subsp ◽  
Homologous Antigen ◽  
High Level

Monoclonal antibody Mn-Cs1 with a high level of sensitivity and specificity for detection and determination of Clavibacter michiganensis subsp. sepedonicus was prepared. Strain C. m. subsp. sepedonicus NCPPB 3467 (as whole cell antigen and extracellular polysaccharides) was used for immunisation of four mice Balb/c. After cloning and verifying, two stable hybridoma clones were gained. One monoclonal antibody, designated Mn-Cs1, was used in all tests. It reacted intensely with extracellular polysaccharides from homologous antigen (&gt; 0.5 mg/ml), weakly with proteins from cell walls (&gt; 200 &mu;g/ml) and with whole homologous antigen (concentration 104&ndash;103 cfu/ml) in DAS-ELISA. Monoclonal antibody Mn-Cs1 showed a high level of specificity. It reacted neither with bacterial strains of closely related subspecies of Clavibacter michiganensis (C. m. subsp. michiganensis and C. m. subsp. insidiosus) nor with the saprophytic bacteria Pseudomonas fluorescens and Pantoea agglomerans.

scholarly journals A New Selective Medium for Isolation of Clavibacter michiganensis subsp. michiganensis from Tomato Plants and Seed

2011 ◽  
Vol 101 (11) ◽  
pp. 1355-1364 ◽  
Author(s):  
Radwan M. Ftayeh ◽  
Andreas von Tiedemann ◽  
Klaus W. E. Rudolph
Keyword(s):  
Bacterial Species ◽  
False Negative ◽  
Selective Medium ◽  
Detection Sensitivity ◽  
Bacterial Canker ◽  
Clavibacter Michiganensis ◽  
Tomato Seed ◽  
Tomato Plants ◽  
Clavibacter Michiganensis Subsp ◽  
Bacterial Canker Of Tomato

A new selective and highly sensitive medium was developed for isolation of Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of bacterial canker of tomato, from seed and latently infected plants. The new medium (BCT) proved to be superior to all published semiselective media for Cmm and is denoted as selective medium because of (i) its mean plating efficiency, amounting to ≤89% within 7 days for all 30 Cmm strains from different sources tested; (ii) the high selectivity, because accompanying bacterial species occurring on tomato plants and seed or bacteria obtained from culture collections were inhibited to an extent of 98 to 100%; and (iii) the remarkable detection sensitivity. Thus, 8 CFU of Cmm in field plant homogenates containing 12,750 CFU of accompanying saprophytes were detected on BCT. Under these extreme conditions, all of the published semiselective media (D2, KBT, D2ANX, SCM, mSCM, CMM1, mCNS, and EPPO) gave false-negative results. Either some media were rather toxic and Cmm growth was also inhibited or the other, less toxic media allowed growth of high numbers of saprophytes, so that Cmm growth was suppressed. Exclusively, BCT also supported growth of the closely related C. michiganensis subsp. insidiosus, nebraskensis, and tessellarius. The new medium is recommended for Cmm detection in tomato seed, and in symptomless tomato plantlets, to improve disease control of bacterial canker of tomato.

scholarly journals Characteristics of polyclonal antisera for detection and determination of Clavibacter michiganensis subsp. insidiosus

2000 ◽  
Vol 36 (No. 2) ◽  
pp. 46-52 ◽  
Author(s):  
B. Kokošková ◽  
I. Pánková ◽  
V. Krejzar
Keyword(s):  
Clavibacter Michiganensis ◽  
Clavibacter Michiganensis Subsp ◽  
Polyclonal Antisera

A rapid selective medium for the determination of trimethylamineoxide-reducing bacteria

1974 ◽  
Vol 14 (8) ◽  
pp. 655-658 ◽  
Author(s):  
J. M. Debevere ◽  
J. P. Voets
Keyword(s):  
Selective Medium ◽  
Reducing Bacteria

Clavibacter michiganensis subsp. sepedonicus. [Distribution map].

2017 ◽  
Author(s):  
Keyword(s):  
Solanum Tuberosum ◽  
New Brunswick ◽  
Prince Edward Island ◽  
Distribution Map ◽  
Clavibacter Michiganensis ◽  
Central Russia ◽  
Clavibacter Michiganensis Subsp ◽  
Northern Russia ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Clavibacter michiganensis subsp. sepedonicus (Spieckermann & Kotthoff) Dye & Kemp. Actinobacteria: Actinomycetales: Microbacteriaceae. Hosts: potato (Solanum tuberosum) and tomato (Solanum lycopersicum). Information is given on the geographical distribution in Europe (Austria, Belarus, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, Germany, Greece, Crete, Hungary, Latvia, Lithuania, Netherlands, Norway, Poland, Romania, Russia, Central Russia, Northern Russia, Siberia, Slovakia, Spain, Sweden, UK and Ukraine), Asia (China, Anhui, Gansu, Hebei, Heilongjiang, Henan, Jiangsu, Ningxia, Shaanxi, Yunnan, Zhejiang, Japan, Kazakhstan, Korea Democratic People's Republic, Korea Republic, Nepal, Pakistan, Taiwan, Turkey and Uzbekistan), North America (Canada, Alberta, British Columbia, Manitoba, New Brunswick, Newfoundland, Nova Scotia, Ontario, Prince Edward Island, Quebec, Saskatchewan, Mexico, USA, Idaho, Kansas, North Dakota and Oregon) and South America (Bolivia).

Factors Affecting Survival of Clavibacter michiganensis subsp. sepedonicus in Water

2004 ◽  
Vol 152 (3) ◽  
pp. 161-168 ◽  
Author(s):  
J. M. Wolf ◽  
J. R. C. M. Beckhoven
Keyword(s):  
Clavibacter Michiganensis ◽  
Factors Affecting ◽  
Clavibacter Michiganensis Subsp
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