Biochemical Characterization of Thylakoids and CF0CF1 Isolated from Chlamydomonas Reinhardtii CW15 and from atpA and atpB Deletion Mutants

1995 ◽  
pp. 1975-1978
Author(s):  
Heike R. Fiedler ◽  
Stefan Leu ◽  
Noun Shavit ◽  
Heinrich Strotmann
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization ◽  
Deletion Mutants

scholarly journals Biochemical Characterization of the Chlamydomonas reinhardtii α-1,4 Glucanotransferase Supports a Direct Function in Amylopectin Biosynthesis

1999 ◽  
Vol 120 (4) ◽  
pp. 1005-1014 ◽  
Author(s):  
Christophe Colleoni ◽  
David Dauvillée ◽  
Gregory Mouille ◽  
Matthew Morell ◽  
Michael Samuel ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization ◽  
Direct Function

scholarly journals Biochemical Characterization of Wild-Type and Mutant Isoamylases of Chlamydomonas reinhardtii Supports a Function of the Multimeric Enzyme Organization in Amylopectin Maturation

2001 ◽  
Vol 125 (4) ◽  
pp. 1723-1731 ◽  
Author(s):  
David Dauvillée ◽  
Christophe Colleoni ◽  
Gregory Mouille ◽  
Matthew K. Morell ◽  
Christophe d'Hulst ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization ◽  
Wild Type ◽  
Enzyme Organization

scholarly journals Genome-wide identification and biochemical characterization of calcineurin B-like calcium sensor proteins in Chlamydomonas reinhardtii

2020 ◽  
Vol 477 (10) ◽  
pp. 1879-1892
Author(s):  
Manoj Kumar ◽  
Komal Sharma ◽  
Akhilesh K. Yadav ◽  
Kajal Kanchan ◽  
Madhu Baghel ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Physiological Processes ◽  
Calcineurin B ◽  
Genome Wide ◽  
Dependent Protein ◽  
Sensor Proteins

Calcium (Ca2+) signaling is involved in the regulation of diverse biological functions through association with several proteins that enable them to respond to abiotic and biotic stresses. Though Ca2+-dependent signaling has been implicated in the regulation of several physiological processes in Chlamydomonas reinhardtii, Ca2+ sensor proteins are not characterized completely. C. reinhardtii has diverged from land plants lineage, but shares many common genes with animals, particularly those encoding proteins of the eukaryotic flagellum (or cilium) along with the basal body. Calcineurin, a Ca2+/calmodulin-dependent protein phosphatase, is an important effector of Ca2+ signaling in animals, while calcineurin B-like proteins (CBLs) play an important role in Ca2+ sensing and signaling in plants. The present study led to the identification of 13 novel CBL-like Ca2+ sensors in C. reinhardtii genome. One of the archetypical genes of the newly identified candidate, CrCBL-like1 was characterized. The ability of CrCBL-like1 protein to sense as well as bind Ca2+ were validated using two-step Ca2+-binding kinetics. The CrCBL-like1 protein localized around the plasma membrane, basal bodies and in flagella, and interacted with voltage-gated Ca2+ channel protein present abundantly in the flagella, indicating its involvement in the regulation of the Ca2+ concentration for flagellar movement. The CrCBL-like1 transcript and protein expression were also found to respond to abiotic stresses, suggesting its involvement in diverse physiological processes. Thus, the present study identifies novel Ca2+ sensors and sheds light on key players involved in Ca2+signaling in C. reinhardtii, which could further be extrapolated to understand the evolution of Ca2+ mediated signaling in other eukaryotes.

scholarly journals Biochemical Characterization of Glutaredoxins from Chlamydomonas reinhardtii Reveals the Unique Properties of a Chloroplastic CGFS-type Glutaredoxin

2008 ◽  
Vol 283 (14) ◽  
pp. 8868-8876 ◽  
Author(s):  
Mirko Zaffagnini ◽  
Laure Michelet ◽  
Vincent Massot ◽  
Paolo Trost ◽  
Stéphane D. Lemaire
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization

scholarly journals Biochemical characterization of the small ubiquitin-like modifiers of Chlamydomonas reinhardtii

Planta ◽  
2010 ◽  
Vol 232 (3) ◽  
pp. 649-662 ◽  
Author(s):  
Yung-Cheng Shin ◽  
Bang-Yu Liu ◽  
Jia-Yun Tsai ◽  
Jiunn-Tzong Wu ◽  
Li-Kwan Chang ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization

Purification and Biochemical Characterization of PSI-LHCI Supercomplex in Chlamydomonas reinhardtii

2008 ◽  
pp. 215-218
Author(s):  
Mariko Sumimoto ◽  
Takahito Onishi ◽  
Jian-Ren Shen ◽  
Yuichiro Takahashi
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization
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