Functional Expression of the β Subunit of the Rhodospirillum Rubrum F1-ATPase.

1995 ◽  
pp. 1991-1994
Author(s):  
L. Nathanson ◽  
Z. Gromet-Elhanan
Keyword(s):  
Rhodospirillum Rubrum ◽  
Functional Expression ◽  
Β Subunit ◽  
F1 Atpase

scholarly journals The Mitochondrial Genome Integrity Gene, MGI1, of Kluyveromyces lactis Encodes the β-Subunit of F1-ATPase

Genetics ◽  
1996 ◽  
Vol 144 (4) ◽  
pp. 1445-1454 ◽  
Author(s):  
Xin Jie Chen ◽  
G Desmond Clark-Walker
Keyword(s):  
Mitochondrial Genome ◽  
Kluyveromyces Lactis ◽  
Three Dimensional ◽  
Genome Integrity ◽  
Dimensional Structure ◽  
Deletion Mutants ◽  
Β Subunit ◽  
Gain Of Function ◽  
F1 Atpase ◽  
Γ Subunit

In a previous report, we found that mutations at the mitochondrial genome integrity locus, MGI1, can convert Kluyveromyces lactis into a petite-positive yeast. In this report, we describe the isolation of the MGI1 gene and show that it encodes the β-subunit of the mitochondrial F1-ATPase. The site of mutation in four independently isolated mgi1 alleles is at Arg435, which has changed to Gly in three cases and Ile in the fourth isolate. Disruption of MGI1 does not lead to the production of mitochondrial genome deletion mutants, indicating that an assembled F1 complex is needed for the “gain-of-function” phenotype found in mgi1 point mutants. The location of Arg435 in the β-subunit, as deduced from the three-dimensional structure of the bovine F1-ATPase, together with mutational sites in the previously identified mgi2 and mgi5 alleles, suggests that interaction of the β- and α- (MGI2) subunits with the γ-subunit (MGI5) is likely to be affected by the mutations.

scholarly journals E. coli F1 -ATPase: Site-directed mutagenesis of the β-subunit

FEBS Letters ◽  
1988 ◽  
Vol 232 (1) ◽  
pp. 111-114 ◽  
Author(s):  
Derek Parsonage ◽  
Susan Wilke-Mounts ◽  
Alan E. Senior
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Β Subunit ◽  
E Coli ◽  
F1 Atpase

Structural energetics of MgADP binding to the isolated β subunit of F1-ATPase from thermophilic Bacillus PS3

2002 ◽  
Vol 408 (2) ◽  
pp. 177-183 ◽  
Author(s):  
Gerardo Pérez-Hernández ◽  
Enrique Garcı́a-Hernández ◽  
Rafael A. Zubillaga ◽  
Marietta Tuena de Gómez-Puyou
Keyword(s):  
Β Subunit ◽  
Thermophilic Bacillus ◽  
F1 Atpase
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