The impact of metal ion chemistry on our understanding of enzymes

1990 ◽  
pp. 227-264
Author(s):  
Donald H. Brown ◽  
W. Ewan Smith
Keyword(s):  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
A. Sukumaran ◽  
S. Pladwig ◽  
J. Geddes-McAlister

Abstract Background Microbial organisms encounter a variety of environmental conditions, including changes to metal ion availability. Metal ions play an important role in many biological processes for growth and survival. As such, microbes alter their cellular protein levels and secretion patterns in adaptation to a changing environment. This study focuses on Klebsiella pneumoniae, an opportunistic bacterium responsible for nosocomial infections. By using K. pneumoniae, we aim to determine how a nutrient-limited environment (e.g., zinc depletion) modulates the cellular proteome and secretome of the bacterium. By testing virulence in vitro, we provide novel insight into bacterial responses to limited environments in the presence of the host. Results Analysis of intra- and extracellular changes identified 2380 proteins from the total cellular proteome (cell pellet) and 246 secreted proteins (supernatant). Specifically, HutC, a repressor of the histidine utilization operon, showed significantly increased abundance under zinc-replete conditions, which coincided with an expected reduction in expression of genes within the hut operon from our validating qRT-PCR analysis. Additionally, we characterized a putative cation transport regulator, ChaB that showed significantly higher abundance under zinc-replete vs. -limited conditions, suggesting a role in metal ion homeostasis. Phenotypic analysis of a chaB deletion strain demonstrated a reduction in capsule production, zinc-dependent growth and ion utilization, and reduced virulence when compared to the wild-type strain. Conclusions This is first study to comprehensively profile the impact of zinc availability on the proteome and secretome of K. pneumoniae and uncover a novel connection between zinc transport and capsule production in the bacterial system.


1996 ◽  
pp. 47-87 ◽  
Author(s):  
Charles W. Bauschlicher ◽  
Stephen R. Langhoff ◽  
Harry Partridge

2019 ◽  
Vol 631 ◽  
pp. A101 ◽  
Author(s):  
Konstantin Herbst ◽  
John Lee Grenfell ◽  
Miriam Sinnhuber ◽  
Heike Rauer ◽  
Bernd Heber ◽  
...  

Context. The first opportunity to detect indications for life outside of the Solar System may be provided already within the next decade with upcoming missions such as the James Webb Space Telescope (JWST), the European Extremely Large Telescope (E-ELT) and the Atmospheric Remote-sensing Infrared Exoplanet Large-survey (ARIEL) mission, searching for atmospheric biosignatures on planets in the habitable zone of cool K- and M-stars. Nevertheless, their harsh stellar radiation and particle environment could lead to photochemical loss of atmospheric biosignatures. Aims. We aim to study the influence of cosmic rays on exoplanetary atmospheric biosignatures and the radiation environment considering feedbacks between energetic particle precipitation, climate, atmospheric ionization, neutral and ion chemistry, and secondary particle generation. Methods. We describe newly combined state-of-the-art modeling tools to study the impact of the radiation and particle environment, in particular of cosmic rays, on atmospheric particle interaction, atmospheric chemistry, and the climate-chemistry coupling in a self-consistent model suite. To this end, models like the Atmospheric Radiation Interaction Simulator (AtRIS), the Exoplanetary Terrestrial Ion Chemistry model (ExoTIC), and the updated coupled climate-chemistry model are combined. Results. In addition to comparing our results to Earth-bound measurements, we investigate the ozone production and -loss cycles as well as the atmospheric radiation dose profiles during quiescent solar periods and during the strong solar energetic particle event of February 23, 1956. Further, the scenario-dependent terrestrial transit spectra, as seen by the NIR-Spec infrared spectrometer onboard the JWST, are modeled. Amongst others, we find that the comparatively weak solar event drastically increases the spectral signal of HNO3, while significantly suppressing the spectral feature of ozone. Because of the slow recovery after such events, the latter indicates that ozone might not be a good biomarker for planets orbiting stars with high flaring rates.


2019 ◽  
Vol 2020 (6) ◽  
pp. 523-531 ◽  
Author(s):  
Yulia S. Kudyakova ◽  
Pavel A. Slepukhin ◽  
Marina S. Valova ◽  
Yanina V. Burgart ◽  
Viktor I. Saloutin ◽  
...  

Metallomics ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 925-935 ◽  
Author(s):  
Rohit Budhraja ◽  
Chang Ding ◽  
Philipp Walter ◽  
Stephan Wagner ◽  
Thorsten Reemtsma ◽  
...  

Absolute metal ion content was determined from whole cells of different microbial species and changes were related to growth conditions and change of encoded genes.


2017 ◽  
Vol 89 (1-2) ◽  
pp. 1-14 ◽  
Author(s):  
Yu Qing Yao ◽  
Kai Chen ◽  
Zi Yi Hua ◽  
Qian Jiang Zhu ◽  
Sai Feng Xue ◽  
...  
Keyword(s):  

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