Photohydrogen Production - Hydrogenases in the Green Alga Scenedesmus Obliquus

1998 ◽  
pp. 1951-1954 ◽  
Author(s):  
Röbbe Wünschiers ◽  
Horst Senger ◽  
Rüdiger Schulz
Keyword(s):  
Green Alga ◽  
Scenedesmus Obliquus

Light-Dependent Hydrogen Production of the Green Alga Scenedesmus obliquus

BioHydrogen ◽  
2007 ◽  
pp. 243-251
Author(s):  
Rüdiger Schulz ◽  
Jörg Schnackenberg ◽  
Kerstin Stangier ◽  
Röbbe Wünschiers ◽  
Thomas Zinn ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Green Alga ◽  
Scenedesmus Obliquus

Isolation and Purification to Apparent Homogeneity of 4,5-Dioxovalerate Aminotransferase from Scenedesmus obliquus Mutant C-2 A′

1988 ◽  
Vol 43 (7-8) ◽  
pp. 563-571 ◽  
Author(s):  
A. Kah ◽  
D. Dörnemann ◽  
H. Senger
Keyword(s):  
Molecular Weight ◽  
Green Alga ◽  
Isoelectric Point ◽  
Pyridoxal Phosphate ◽  
Scenedesmus Obliquus ◽  
Ph Optimum ◽  
Isolation And Purification ◽  
Apparent Homogeneity ◽  
Unicellular Green Alga ◽  
Glyoxylate Aminotransferase

In the present paper the purification of a specific 4,5-dioxovalerate transaminase from pigment mutant C-2 A′ of the unicellular green alga Scenedesmus obliquus to apparent homogeneity is described. The newly isolated enzyme ʟ-glutamate: 4,5-dioxovalerate aminotransferase is not identical with ʟ-alanine: 4,5-dioxovalerate aminotransferase (EC 2.6.1.43) and ʟ-alanine: glyoxylate aminotransferase (EC 2.6.1.44). A procedure for the purification is described and the resulting homogeneous protein is characterized by its Kᴍ-values for oxo-substrates and amino donors, its pyridoxal phosphate requirement, reversability of the catalysis, pH-optimum, isoelectric point and its molecular weight.

Purification and Characterization of Cytochrome c6 from the Unicellular Green Alga Scenedesmus obliquus

1997 ◽  
Vol 52 (11-12) ◽  
pp. 740-746 ◽  
Author(s):  
Röbbe Wünschiers ◽  
Thomas Zinn ◽  
Dietmar Linder ◽  
Rüdiger Schulz
Keyword(s):  
Cytochrome C ◽  
Green Alga ◽  
Scenedesmus Obliquus ◽  
Terminal Sequence ◽  
Ammonium Sulfate Precipitation ◽  
Purification And Characterization ◽  
Unicellular Green Alga ◽  
Sds Page ◽  
Monoraphidium Braunii

Abstract Purification of a soluble cytochrome c6 from the unicellular green alga Scenedesmus obliquus by a simple and rapid method is described. The purification procedure includes ammonium sulfate precipitation and non-denaturating PAGE. The N-terminal sequence of the first 20 amino acids was determined and shows 85% similarity and 75% identity to the sequence of cytochrome c6 from the green alga Monoraphidium braunii. The ferrocyto-chrome shows typical UV/VIS absorption peaks at 552.9, 521.9 and 415.7 nm. The apparent molecular mass was estimated to be 12 kD a by SDS-PAGE. EPR-spectroscopy at 20K shows resonances indicative for two distinct low-spin heme forms.

Sign in / Sign up

Export Citation Format

Share Document